喉癌组织中LKB1与VEGF -C表达的相关性研究

目的:检测喉癌组织中抑癌基因LKB1和血管内皮生长因子VEGF-C的表达情况,探讨VEGF-C在喉鳞状细胞癌、正常组织中的表达关系.方法:选取喉癌患者40例喉癌组织为实验组,40例癌旁正常组织(距肿瘤边缘＞5mm,且病理证实为正常组织)为对照组.采用逆转录聚合酶链反应(RT-PCR)技术分析两组之间LKB1mRNA和VEGF-CmRNA的表达情况.结果:LKB1在癌组织中阳性表达率低于癌旁组织(P＜0.05),VEGF-C在癌组织中阳性表达率高于癌旁组织(P＜0.05),均与组织分化程度有相关性(P＜0.05).LKB1与VEGF-C呈直线负相关.结论:在喉鳞状细胞癌发生的过程中,LKB1和VEGF -C发生异常表达,且与喉鳞状细胞癌的进展有关.

Expression and Significance of LKB1and VEGF - C in Laryngeal Carcinoma

Objective：To detect the expressions of LKB1 and blood vessels endothelial growth factor （VEGF- C） in laryngeal squamous cell carcinoma （LSCC）, to explore the interrelationship and their relation in LSCC. Methods：40 samples of LSCC tissues were selected as the experimental group. The expression of LKB1 and VEGF - C in all the tissues were detected by RT - PCR and clinical medical data were analyzed. Results：LKB1 was related to the progression degree of stain in laryngeal squamous cell carcinoma and was differ- ent from that in the normal laryngeal tissues in the molecular level （P 〈 0.05 ） . The positive expression of LKB1 was related to the pathologic classifications in laryngeal carcinoma（P 〈 0.05 ） ;VEGF-C was related to the progression degree of stain in laryngeal squamous cell carcinoma and was different from that in the normal laryngeal tissues in the molecular level （P 〈 0.05 ）. The positive expression of VEGF - C was related to the pathologic classifications in laryngeal carcinoma （P 〈 0.05 ）. LKB1 and VEGF - C had a negative correlation in laryngeal cancer. Conclusions：Laryngeal cancer with LKB1 and VEGF -C expressions is prone to abnormity expressions, and there are different actions in the 1 arvngeal cancer.