苦参碱联合阿霉素对乳腺癌细胞增殖及凋亡的影响

目的:探讨苦参碱(MA)对阿霉素(ADM)抗肿瘤作用的影响,以期为乳腺癌的治疗提供新的靶点.方法:MTT法分别检测不同浓度(0,0.125,0.25,0.5,1.0,2 g/L) MA、不同浓度(0,0.625,1.25,2.5,5,10 mg/L) ADM以及ADM(0.4 mg/L)与MA(0.5 g/L)合用对乳腺癌细胞株MCF -7的增殖抑制作用.溴化丙啶(PI)单染检测MA(0.5 g/L)联合ADM(0.4 mg/L)诱导MCF -7细胞凋亡的影响.结果:随着药物作用时间以及药物浓度的增加,MA、ADM以及两种合用对MCF -7细胞增殖的抑制作用逐渐增强.0.5 g,/LMA诱导MCF -7细胞凋亡率低,仅为12.58％,0.4 mg/LADM诱导MCF -7细胞凋亡率为35.12％；而0.5 g/LMA与0.4ng/LADM联合使用诱导MCF -7细胞凋亡率为68.11％,明显高于ADM或MA单独使用.结论:MA、ADM及二者联用对MCF -7细胞的增殖抑制作用具有时间依赖性和剂量依赖性.MA本身诱导MCF -7细胞凋亡率低,但它能增加ADM诱导MCF -7细胞的凋亡率.MA可作为抗癌药的化疗增敏剂.

Effect of Matrine Combined with Adriamycin on the Proliferation and Apoptosis of Breast Cancer Cells

Objective：To determine whether matrine （MA） could synergize with chemotherapeutic agent Adriamycin（ADM） which was commonly used in breast cancer treatment in order to find new targets for human breast cancer chemotherapy. Methods： MCF - 7 breast cancer cells were incubated with different concentrations of ADM （0.625, 1.25, 2.5, 5.0 and 10 m9/L） with or without MA （0,0. 125,0.25,0.5,1.0,2 g/L）. Cell viability was measured by the MTT assay. MCF- 7 breast cancer cells were treated with MA（0.5 g/L） and ADM（O. 4 mg/L）. Then apoptotic cells were measured by propidium iodide （PI） staining in Flow Cytometry （FCM）. Results：The pro- liferation of MCF - 7 breast cancer cells could be inhibited in different concentration of MA combined with ADM or alone . The inhibition was enlarged with the increasing concentration of drugs and prolonged with increasing time of the cells exposed to drugs. Apoptosis of MCF - 7 breast cancer cells was induced by MA, ADM and MA ＋ ADM . The apoptosis rates were 12.5% , 35.12% and 68.11% respectively. Combination of MA and ADM induced a further increase of apoptosis rate. Conclusions ： The proliferation of MCF - 7 breast cancer cells can be inhibited by MA, ADM and MA ＋ ADM . This inhibition shows the dose - and exposing time - dependence. The apoptosis rate of MCF - 7 breast cancer cells induced by MA is low, but it can enlarge the apoptosis rate induced by ADM.