Ag43／FGFR-1嵌合蛋白疫苗抗小鼠纤维肉瘤血管生成的实验研究

目的：利用Antigen43（Ag43）／成纤维细胞生长因子I型受体（FGFR一1）重组嵌合蛋白作为疫苗，了解其是否具有抑制小鼠肿瘤生长的作用，并初步探讨其作用机理。方法：40只BALB／c雌性小鼠接种MethA细胞后第7天，随机分为Ag43／FGFR．1蛋白免疫组（AF组）、A酗3蛋白免疫组（Ag43组）、FGFR—1蛋白免疫组（FGFR一1组）和生理盐水对照组（NS组）4组，每组10只，观察免疫治疗后的荷瘤小鼠肿瘤体积和生存曲线，分别用免疫组织化学方法检测肿瘤组织微血管密度（MVD），免疫印迹（Western blot）方法检测抗自身FGFR—1的抗体，酶联免疫斑点试验（ELISPOT）检测分泌抗自身FGFR-1抗体的B淋巴细胞的数量。结果：Ag43／FGFR-1组与FGFR-1蛋白、A甜3蛋白、生理盐水对照组肿瘤组织MVD计数分别为11．9±2．3、59．6±3．8、60．6±1．2和61．9±3．4（P〈0．01）；与对照组相比，Ag43／FGFR-1组肿瘤体积明显变小（P〈0．01），存活时间明显延长（P〈0．01），血清中发现含有抗自身FGFR-1的抗体且发现小鼠脾脏中分泌抗自身FGFR-1抗体的B淋巴细胞数目明显增多（P〈0．01）。结论：Ag43／FGFR-1蛋白质疫苗能够诱导荷瘤鼠产生特异性免疫反应，从而抑制肿瘤血管生成和肿瘤的生长。

Study on the anti-angiogenesis mechanism induced by a Ag43/FGFR1protein vaccine in a mouse Meth A fibrosarcoma model

Objective: To explore the anti-tumor effect of immunotherapy with a recombinant Ag43/FGFR-1 chimeric protein vaccine in a mouse Meth A fibrosarcoma model.Tumor volume and survival rate of the mice were observed in 3-day intervals.Methods: Microvessel density(MVD) was detected by immunohistochemistry.Autoantibodies against self-FGFR-1 were detected by Western blot.The anti-FGFR-1 antibody-producing B cells(APBCs) were detected by enzyme-linked immunospot(ELISPOT) assay.Results: MVD was significantly lower in...