生长抑素类似物诱导人胆管癌SK-ChA-1细胞调亡及调亡相关基因表达的研究

目的：探讨生长抑素类似物SMS201－995（SMS）抑制人胆管癌生长时对SK－ChA-1细胞凋亡调控基因bcl-2和bax的作用。方法：用DNA凝胶电泳和流式细胞仪检测Annexin V标记凋亡细胞技术研究SMS诱导细胞凋亡的情况；用免疫组化和原位杂交分析SMS凋亡调控基因bcl-2和bax的影响。结果：SMS处理SK－ChA-1细胞后，Annexin V标记的凋亡细胞增多，DNA凝胶电泳显示典型的凋亡和梯状图谱，同时SMS可使细胞bcl-2蛋白表达下降，使bax蛋白和mRNA表达升高。结论：SMS能诱导SK－ChA-1细胞发生凋亡，bax和bcl-2凋亡基因介导了SMS对SK－ChA-1细胞凋亡的发生。

Apoptosis of human cholangiocarcinoma cell line SK-ChA-1 induced by somatostatin analogues and its related gene expressions

Objective To investigate the role of somatostatin analogue (SMS201 995, SMS) on apoptosis of human cholangiocarcinoma SK ChA 1 cells and on apoptosis regulated gene bcl 2 and bax since the analogue inducing the inhibition of human cholangiocarcinoma growth. Methods DNA gel electrophoresis, flow cytometry with Annexin V as marker were used to detect the apoptosis of SK ChA 1 cells. The expressions of bcl 2 and bax were studied with SABC immunohistochemistry and in situ hybridization. Results The annexin V positive cells were increased after SMS treatment and showed a typical ladder pattern as apoptosis feature on gel. Besides, SMS inhibited bcl 2 protein expression and enhanced bax protein and mRNA expressions. Conclusion SMS can induce SK ChA 1 cell apoptosis by means of affecting bcl 2 and bax gene expressions.