多烯紫杉醇和姜黄素联用对人肺腺癌A549细胞增殖和凋亡的影响

目的研究姜黄素和多烯紫杉醇对肺腺癌A549细胞增殖及调亡的影响，并探讨两种药物的合用是否可增强抗癌作用及降低不良反应。方法体外培养肺腺癌细胞系A549细胞，采用 MTT 法计算细胞抑制率；Giemsa 染色法观察细胞形态学变化；流式细胞仪分析细胞的凋亡率。结果经MTT法检测显示，姜黄素能明显抑制A549细胞的生长，多烯紫杉醇50 μg/ml与姜黄素5、10、20 μmol/L联用对A549细胞的抑制作用明显大于两药单用时的抑制作用（P<0.01）；Giemsa染色显示多烯紫杉醇与姜黄素联用后诱导细胞凋亡的作用增强，可见典型的凋亡小体；流式细胞仪结果表明姜黄素能增加多烯紫杉醇的细胞凋亡率，其作用呈剂量依赖性。结论姜黄素与多烯紫杉醇可协同抑制人肺腺癌A549细胞的增殖；二者联合化疗有增效减毒作用。

Effect of Combination of Docetaxel with Curcumin on Proliferation and Apoptosis of Human Lung Adenocarcinoma A549 Cell

Objective To study the effect of docetaxel and curcumin on proliferation and apoptosis of human lung adenocarcinoma cell line A549, and to investigate whether the combination of docetaxel with curcumin can strengthen antitumous effect and decrease toxic side effect or not. Methods The lung adenocarcinoma cell line A549 was cultured in vitro. The growth inhibition ratio, morphologic changes and apoptosis index were measured by MTT test, Giemsa staining and flow cytometry, respectively. Results MTT results suggested that curcumin effectively inhibited the proliferation of A549 cells. Further more the inhibitory ratio of the combination of curcumin with docetaxel group was statistically higher than the curcumin group (P<0.01).The apoptosis body could also be found by Giemsa staining. It was confirmed by FACS that docetaxel and curcumin can both induce cell apoptosis. When the two drugs were com-bined,the inductive effect on cell apoptosis became more obvious and was in concentration-dependent patterns. Conclusion Curcumin can synergistically inhibit the proliferation of A549 cell with the combination of docetaxel. The combination of curcumin and docetaxel enhanced the effect and decrease the attenuation in chemotherapy.