HPLC法同时测定复方明目胶囊中绿原酸和秦皮甲素的含量

目的:建立以高效液相色谱法同时测定复方明目胶囊中绿原酸和秦皮甲素含量的方法。方法:色谱柱为Zorbax-C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(用三乙胺调pH4.5)=8∶92,检测波长为327nm,流速为0.8mL·min-1,柱温为25℃。结果:绿原酸进样量在1.00~6.00μg范围内与峰面积积分值呈良好线性关系(r=0.9993),平均加样回收率为98.5%,RSD=1.04%(n=6);秦皮甲素进样量在1.60~12.32μg范围内与峰面积积分值呈良好线性关系(r=0.9996),平均加样回收率为99.2%,RSD=0.76%(n=6)。结论:本方法分离度高、专属性强、干扰小、重现性好,适用于复方明目胶囊的质量控制。

Simultaneous Determination of Chlorogenic Acid and Aesculin Contents in Compound Mingmu Capsules by HPLC

OBJECTIVE:To develop an HPLC method for the simultaneous determination of Photogenic acid and Aesculin in Compound mingmu capsules. METHODS: The analytical column was Zorbax-C18(250 mm×4.6 mm,5 μm) and the mobile phase consisted of acetonitrile-0.1% orthophosphoric acid solution (8∶92,with pH=4.5 adjusted by triethylamine) at a flow rate of 0.8 mL·min-1. The detector wavelength was set at 327 nm. The column temperature was 25 ℃.RESULTS: The calibration curve were linear over the range of 1.00～6.00 μg for Chlorogenic acid (r=0.999 3) with an average recovery rate of 98.5%(RSD=1.04%,n=6); and the linear range of Aesculin was 1.60～12.32 μg(r=0.999 6) with an average recovery of 99.2%(RSD=0.76%,n=6). CONCLUSION: The method is of high resolution, strong specificity and good reproducibility yet with little interference, thus suitable for the quality control of Compound mingmu capsules.