| 免疫球蛋白IgG对H_2O_2所致内皮细胞损伤的抑制作用及其机制 |
| |
| 引用本文： | 常贺,李刚,王焱,邹军. 免疫球蛋白IgG对H_2O_2所致内皮细胞损伤的抑制作用及其机制[J]. 中国生化药物杂志, 2012, 33(6): 713-716,724 |
| |
| 作者姓名： | 常贺 李刚 王焱 邹军 |
| |
| 作者单位： | 1. 厦门大学 附属中山医院 心内科,厦门心脏中心 福建厦门,361004
2. 厦门大学医学院 福建厦门361005 |
| |
| 基金项目： | 福建省科技计划重点项目，国家自然科学基金资助项目，福建省自然科学基金资助项目 |
| |
| 摘 要： | 目的探讨免疫球蛋白IgG对H2O2诱导的内皮细胞的黏附分子和趋化因子的表达及作用机制。方法IgG和H2O2加入内皮细胞中孵育2 h,应用RT-PCR以及实时定量RT-PCR检测黏附因子(ICAM-1、VCAM-1、E-se-lectin)及趋化因子(MCP-1、CXCL-1、MIP-2)的mRNA及蛋白表达;进一步应用Western blot检测IgG对H2O2诱导的p38、ERK1/2和JNK1/2的磷酸化情况。结果 H2O2可显著诱导黏附分子(ICAM-1、VCAM-1和E-selectin)、趋化因子(MCP-1、CXCL-1、MIP-2)的表达,而IgG对H2O2诱导的这些因子的表达有抑制作用;且IgG可抑制H2O2诱导的p38、JNK1/2和ERK1/2的磷酸化。结论 IgG对H2O2诱导的内皮细胞黏附分子及趋化因子表达的抑制作用可能通过抑制p38、JNK1/2、ERK1/2的信号通路实现,这可能是IgG调节内皮细胞炎症的机制之一。
|
| 关 键 词： | 免疫球蛋白 H2O2 内皮细胞 趋化因子 |
Effect of immunoglobulin IgG on H2O2-induced endothelial cells and possible mechanism |
| |
| CHANG He , LI Gang , WANG Yan , ZOU Jun. Effect of immunoglobulin IgG on H2O2-induced endothelial cells and possible mechanism[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(6): 713-716,724 |
| |
| Authors: | CHANG He LI Gang WANG Yan ZOU Jun |
| |
| Affiliation: | 1.Devision of Cardiology,the Affilated Zhongshan Hospital of Xiamen University,Xiamen Heart Center, Xiamen 361004,China;2.Medical School of Xiamen University,Xiamen 361005,China) |
| |
| Abstract: | Purpose To explore the effect of immunoglobulin(IgG) on the expression of adhesion molecules and chemokines in H2O2-induced endothelial cells(ECs) and possible mechanism.Methods Different concentrations IgG and H2O2 were added to ECs for 2 h.The levels of adhesion molecules(ICAM-1,VCAM-1,E-selectin) and chemokines(MCP-1,CXCL-1,MIP-2) were determined by RT-PCR and quantitative RT-PCR.Furthermore the effect of IgG on the expression of p38,JNK1/2,ERK1/2 in H2O2-induced ECs was determined by Western blot analysis.Results IgG dose-dependently inhibited the production of adhesion moleculus(ICAM-1,VCAM-1,E-selectin) and chemokines(MCP-1,CXCL-1,MIP-2) in the activated ECs induced by H2O2.Conclusion The inhibitory effect of IgG on adhesion molecules,chemokines expression induced by H2O2 in ECs might be mediated by p38、JNK1/2、ERK1/2 signaling pathways. |
| |
| Keywords: | immunoglobulin H2O2 endothelial cells chemokines |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
