| 高效液相色谱法测定麝香风湿片中乌头碱的含量 |
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| 引用本文： | 李明梅. 高效液相色谱法测定麝香风湿片中乌头碱的含量[J]. 中国生化药物杂志, 2012, 33(6): 836-838 |
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| 作者姓名： | 李明梅 |
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| 作者单位： | 盐城卫生职业技术学院,江苏盐城,224005 |
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| 摘 要： | 目的建立麝香风湿片中乌头碱含量的高效液相色谱测定方法。方法采用Agilent C18柱(4.6 mm×150 mm,5μm);流动相:甲醇-0.05%磷酸溶液(含0.04%三乙胺)(68∶32);流速0.8 mL/min;检测波长:235 nm;柱温:30℃;进样量:10μL。结果乌头碱在0.200 6～20.06μg/mL浓度范围与峰面积呈良好线性关系,r=0.999 9,最低检测限约为2 ng,平均回收率为97.7%。结论此方法具有快速、简便、灵敏、准确等特点,适合于麝香风湿片中乌头碱的含量测定。
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| 关 键 词： | 麝香风湿片 乌头碱 含量测定 高效液相色谱法 |
Research on the HPLC method for determining the content of aconitine in Shexiang Fengshi tablets |
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| LI Ming-mei. Research on the HPLC method for determining the content of aconitine in Shexiang Fengshi tablets[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(6): 836-838 |
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| Authors: | LI Ming-mei |
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| Affiliation: | LI Ming-mei(Yancheng Health Vocational Technical College,Yancheng 224005,China) |
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| Abstract: | Purpose To develop a method for deteminating aconitine in Shexiang Fengshi tablets by HPLC.Methods The Agilent C18(4.6 mm×150 mm,5 μm) was used.With a mobile phase of methanol-0.05% phosphoric acid solution(contained 0.04% triethylamine)(68∶ 32),the flow rate was 0.8 mL/min,the detection wavelength was 235 nm,the column temperature was 30 ℃,and the injection was 10 μL.Results The linearity of peak area was good in the range of 0.200 6-20.06 μg/mL(r=0.999 9).The average recovery was 97.7%.Conclusion This method is convenient and accurate.The results of experiment were satisfactory. |
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| Keywords: | Shexiang Fengshi tablet aconitine determination HPLC |
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