| 兔心肌中次黄嘌呤纯化工艺的优化 |
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| 引用本文： | 宋艳,王智鼎,李岳飞,邱岚,姜秀梅,孙德军. 兔心肌中次黄嘌呤纯化工艺的优化[J]. 中国生化药物杂志, 2012, 33(6): 839-841 |
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| 作者姓名： | 宋艳 王智鼎 李岳飞 邱岚 姜秀梅 孙德军 |
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| 作者单位： | 1. 吉林大学 再生医学科学研究所 生物技术药物教研室,吉林长春,130021
2. 吉林省辉发制药股份有限公司,吉林辉南,135100 |
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| 基金项目： | 国家高技术研究发展计划(863) |
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| 摘 要： | 目的探索从兔心肌中提取次黄嘌呤的最佳工艺。方法比较三氟乙酸法、酶解法、酸碱法和醇沉法提取的次黄嘌呤的产率和纯度,得到优化制备工艺。结果利用醇沉法,向细胞充分破碎后的匀浆液中加入4倍体积的纯水,超声提取,向提取液中加入乙醇至浓度60%,置60℃水浴反应120 min,提取的次黄嘌呤产率为0.83 mg/g,纯度为82.4%。结论经过纯化工艺比较,醇沉法提取的次黄嘌呤的含量和纯度优于其他方法。
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| 关 键 词： | 次黄嘌呤 纯化 工艺 优化 |
Optimization of rabbit myocardial hypoxanthine purification process |
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| SONG Yan , WANG Zhi-ding , LI Yue-fei , QIU Lan , JIANG Xiu-mei , SUN De-jun. Optimization of rabbit myocardial hypoxanthine purification process[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(6): 839-841 |
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| Authors: | SONG Yan WANG Zhi-ding LI Yue-fei QIU Lan JIANG Xiu-mei SUN De-jun |
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| Affiliation: | 1(1.Department of Biomedicine,Institute of Frontier Medical Sciences,Jilin University, Changchun 130021,China;2.Huifa Pharmaceutial Co.,Ltd.,Jilin Province,Huinan 135100,China) |
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| Abstract: | Purpose To investigate the content of the different extraction methods of preparation of rabbit myocardial tissue fluid hypoxanthine,to determine the hypoxanthine optimal purification process.Methods Determination of the Trifluoroacetic acid method,enzymatic,ethanol and acid-base extraction hypoxanthine content and purity.Results The tissue fluid too the cells fully broken in four times the volume of pure water,adding ethanol to a concentration of 60% water bath at 60 ℃ for 2 h,extracted hypoxanthine content of 1.410 mg/mL.Conclusion The purification process,compared to the content and purity of the alcohol precipitation extracted hypoxanthine is superior to other methods. |
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| Keywords: | hypoxanthine purification process optimization |
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