| Box-Behnken实验设计法优化胸腺五肽脂质体的处方工艺 |
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| 引用本文： | 张冕,王德堂,刘娥. Box-Behnken实验设计法优化胸腺五肽脂质体的处方工艺[J]. 中国生化药物杂志, 2012, 33(6): 808-810 |
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| 作者姓名： | 张冕 王德堂 刘娥 |
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| 作者单位： | 1. 荆楚理工学院化工与药学院,湖北荆门,448000
2. 荆楚理工学院医学院,湖北荆门,448000 |
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| 基金项目： | 湖北省荆门市科技局引导计划项目"胸腺五肽脂质体工艺研究" |
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| 摘 要： | 目的优化胸腺五肽脂质体的处方工艺。方法采用三因素三水平的Box-Behnken实验设计,利用响应面法对影响胸腺五肽脂质体包封率的主要因素进行考察,以包封率为响应值,建立相应的二项式数学模型优化处方。结果最优处方是脂质体膜材料中磷脂与胆固醇的质量比为17∶5,磷脂与药物的质量比为17∶2,有机相与水相的体积比为3∶1。包封率的预测值与理论值偏差较小。结论 Box-Behnken实验设计法可用于胸腺五肽脂质体处方的优化,经优化的处方工艺稳定可行。
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| 关 键 词： | 胸腺五肽 脂质体 响应面法 Box-Behnken实验设计 |
Optimization of thymopentin liposome formulation by Box-Behnken experimental design |
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| ZHANG Mian , WANG De-tang , LIU E. Optimization of thymopentin liposome formulation by Box-Behnken experimental design[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(6): 808-810 |
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| Authors: | ZHANG Mian WANG De-tang LIU E |
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| Affiliation: | 1(1.School of Chemical Engineering and Pharmacy,2.Medical School,Jingchu University of Technology, Jingmen 448000,China) |
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| Abstract: | Purpose To optimize the formulation of thymopentin liposome.Methods The independent variables were investigated by using a three-factor,three-level Box-Behnken experimental design and response surface methodology.The response variable was encapsulation efficiency.The data were imitated using multi-linear equation and second-order polynomial equation.Results The optimized formulation was as follows:the quality rate of phospholipid and cholesterol was 17∶ 5,the quality rate of phospholipid and drug was 17∶ 2,the volume rate of organic phase and aqueous phase was 3∶ 1.Deviation between observed and predicted values were small.Conclusion The Box-Behnken experimental design is suitable for optimizing the formulation of thymopentin liposome.The optimized formulation is stable and feasible. |
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| Keywords: | thymopentin liposome response surface methodology Box-Behnken experimental design |
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