白英抗肿瘤活性部位筛选

目的研究白英乙醇提取物及其不同溶剂萃取部分对肿瘤细胞的体外抑制作用,筛选白英抗肿瘤活性部位。方法采用四甲基偶氮唑盐(MTT)比色法。结果白英乙醇提物、乙酸乙酯部位、正丁醇部位对肿瘤细胞均有不同程度的抑制作用;乙酸乙酯部位对A375细胞和Hela细胞的抑制作用较强,IC50 值分别为9 6 9mg·L-1和2 0 90mg·L-1;正丁醇部位对A375细胞和Hela细胞的抑制作用次之,IC50 值分别为31 72mg·L-1和6 4 4 2mg·L-1;水溶性部位对A375细胞和Hela细胞无抑制作用。结论乙酸乙酯部位、正丁醇部位有一定的细胞毒作用,初步确定为白英抗肿瘤活性部位。

Preliminary study on the anti-tumor effect of the extracts of Solanum lyratum Thunb.

Objective To estimate the anti-tumor activity of the extracts of Solanum lyratum Thunb.Methods MTT assay was applied to screen the anti-tumor effect of the extracts of Solanum lyratum on different tumor cell lines in vitro.Results Different extracts had variable inhibitory effect on different tumor cell lines.Ethyl acetate part had remarkable anti-tumor activity in vitro,its IC 50 against A375 and Hela cell lines was 9.69 and 20.90 mg·L~ -1 ,respectively.n-Butanol part provided some inhibitory effect on A375 and Hela cell lines,with IC 50 31.72 and 64.42 mg·L~ -1 ,respectively.The water-soluble part had no effect on different tumor cell lines.Conclusions Ethyl acetate part and n-butanol part,which are considered as active parts of Solanum lyratum Thunb.,have considerable cytotoxin effect.