Whole-cell recordings of voltage-gated Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons |
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Authors: | Shuyun Huang Qing Cai Weitian Liu Xiaoling Wang and Tao Wang |
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Institution: | Shuyun Huanga,Qing Caia,Weitian Liub,Xiaoling Wanga,Tao Wanga aDepartment of physiology,Tianjin University of Traditional Medicine,Tianjin 300193,China bDepartment of cerebral surgery,People Hospital of FengRun,Tangshan 064000,China |
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Abstract: | Objective:To record Calcium, Potassium and Sodium currents in acutely isolated hippocampal pyramidal neurons. Methods:Hip-pocampal CA3 neurons were freshly isolated by 1 mg protease/3 ml SES and mechanical trituration with polished pipettes of progressively smaller tip diameters. Patch clamp technique in whole-cell mode was employed to record voltage-gated channel currents. Results:The procedure dissociated hippocampal neurons, preserving apical dendrites and several basal dendrites, without impairing the electrical characteristics of the neurons. Whole-cell patch clamp configuration was successfully used to record voltage-gated Ca2+ currents, delayed rectifier K+ current and voltage-gated Na+ currents. Conclusion:Protease combined with mechanical trituration may be used for the dissociation of neurons from rat hippocampus. Voltage-gated channels currents could be recorded using a patch clamp technique. |
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Keywords: | patch clamp hippocampus voltage-gated channels whole-cell |
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