细胞间粘附分子-1在内耳免疫反应中的表达

目的探讨细胞间粘附分子１（ｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１，ＩＣＡＭ１）在内耳免疫反应中的作用。方法采用钥孔血蓝蛋白激发已全身致敏的２６只大鼠内耳，诱发其内耳免疫反应，然后通过免疫组化技术检测内耳的ＩＣＡＭ１的表达。结果内耳激发后６小时，螺旋轴静脉及其回流小静脉即有ＩＣＡＭ１表达，１２小时内淋巴囊及其囊周区出现ＩＣＡＭ１表达，在２４～４８小时内耳各部位ＩＣＡＭ１表达达最高峰。７２小时各种炎性细胞浸润到内耳的各个部位。随后ＩＣＡＭ１表达逐渐减弱，２８天完全消失。结论内耳免疫反应时，ＩＣＡＭ１在炎性细胞从循环系统进入内耳的过程中发挥着重要作用，调控ＩＣＡＭ１表达的细胞因子也可能还来自内淋巴囊以外的其他细胞。

Expression of intercellular adhesion molecule 1 in immune response of the inner ear

Objective To understand the role of intercellular adhesion molecule 1(ICAM 1) in the immune response of the inner ear. Methods Inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine(KLH)into the scala tympani of animals who had been systemically sensitized.Then the expression of ICAM 1 in the inner ear was examined by immunohistochemistry. Results ICAM 1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules(CV) as early as 6 hours postchallenge. Expression of ICAM 1 was observed on the epithelium of the endolymphatic sac(ES) and perisaccular region at 12 hours. The intensity of ICAM 1 staining reached a maximum by 24 or 48 hours in these sites of the inner ear. By day 28, most specimens were devoid of significant staining for ICAM 1. Conclusion The study elucidates the important role of adhesion molecules in extravasation of inflammatory cells from the systemic circulation during an inner ear immune response. It also shows that cytokines controlling expression of adhesion molecules may be released by cells located outside of ES besides those cells in the ES.