羊膜移植抑制兔角膜碱烧伤后新生血管增殖的研究

目的　比较采用保存羊膜和新鲜羊膜移植抑制角膜碱烧伤后新生血管增殖的效果 ;探讨应用保存人羊膜移植防治角膜碱烧伤后新生血管的手术时机。方法　制备角膜碱烧伤后新生血管增殖的动物模型 ;2 2只家兔 ( 2 2眼 )随机分为4组 :A组 ( 4眼 )作为对照组 ;B组 ( 6眼 )在碱烧伤的急性期行新鲜羊膜移植 ;C组 ( 6眼 )在急性期行保存人羊膜移植 ;D组 ( 6眼 )在瘢痕期行保存人羊膜移植。应用计算机彩色图像处理系统测定角膜新生血管面积。结果　 3个移植组和对照组比较 ,角膜新生血管面积的差异均有显著性意义 (P <0 0 1) ;B组与C组比较无显著性差异 (P >0 0 5 ) ;C组的新生血管面积明显少于D组 (P <0 0 1)。结论　保存羊膜和新鲜羊膜移植均能有效地抑制角膜碱烧伤后新生血管的增殖 ,治疗效果无显著性差异 ;在角膜碱烧伤的急性期施行羊膜移植防治新生血管增殖的效果要优于在瘢痕期手术。

Inhibition of corneal neovascularization induced with alkali burns in rabbits by transplantation human amniotic membrane

Objective To compare the therapeutic effects of preserved and fresh amniotic membrane transplantation (AMT,F- AMT) on treating corneal neovascularization after corneal alkali burns,and determine the surgical timing of AMT. Methods Corneal neovascularization was induced in rabbits with 1 mol/L sodium hydroxide.Twenty-two healthy rabbits were assigned to four groups in random order.Group A (4 rabbits) served as control,group B (6 rabbits) received F-AMT,group C (6 rabbits) received AMT at acute stage of chemical burns and group D was treated with AMT at cicatricial stage of chemical burns.All the rabbits were followed-up for 2 months.The area of new vessel was measured by a personal computer with digital imaging system. Results The neovascularization area in three AMT groups was statistically different in comparison with control group (P<0.05);there was no significant difference between group B and group C in neovascularization area (P>0.05).The neovascularization area of group C was less than that of group D (P<0.01). Conclusion Both AMT and F- AMT can inhibit the proliferation of corneal neovascularization after alkali burns,and these surgical outcomes are similar.The best surgical timing of AMT is at acute stage of alkali burns.