缺氧、bFGF和VEGF对牛视网膜血管内皮细胞和周细胞增殖的影响

目的　探讨缺氧和细胞生长因子对视网膜血管内皮细胞和周细胞增殖的影响。方法　 ( 1)采用细胞克隆、机械除杂法培养视网膜血管内皮细胞和周细胞。 ( 2 )四唑盐比色试验 (MTT)和细胞计数法描绘正常和缺氧状态下牛视网膜血管内皮细胞和周细胞的增殖生长曲线。 ( 3 )加入碱性成纤维细胞生长因子 (bFGF)和血管内皮细胞生长因子(VEGF)后用MTT法观察它们对内皮细胞增殖的影响。结果　 ( 1)缺氧组牛视网膜血管内皮细胞在第 4、5d数目较正常组显著增加 (P <0 0 5 ) ,周细胞则在 4～ 7d数目明显增多。 ( 2 )加入VEGF可使四甲基偶氮唑盐 3 ( 4 ,5 dimethylthia zolzyl) 2 ,5 diphenyltetrazo diumbromide ,MTT]值达到 2 1786,加入bFGF可使MTT值达到 2 9897,加入VEGF和bFGF使MTT值达到 2 943 1,而对照组仅为 1 0 13。结论　 ( 1)缺氧环境能刺激培养的牛视网膜血管内皮细胞和周细胞的增殖。( 2 )VEGF和bFGF能促进视网膜血管内皮细胞增殖 ,且bFGF促增殖的能力更强 ,但两者相加并不能增加促增殖作用。

Effect of hypoxia,bFGF and VEGF on proliferation of bovine microvascular endothelial cell and pericyte

ObjectiveTo investigate the effects of hypoxia and growth factor on proliferation of bovine retina microvascular endothelial cell and pericyte.MethodsBovine retina microvascular endothelial cell and pericyte were obtained by using cell cloning separating and pericyte weeding.MTT(3-(4,5-dimethylthia-zolzyl)-2,5-diphenyl tetrazo-dium bromide])and cell counting were used to define growth curve of retina microvascular endothelial cell and pericyte under normal and hypoxic condition.Effects of bFGF and VEGF on the proliferation of bovine retina microvascular endothelial cell were observed using MTT.ResultsUnder hypoxic condition,the numbers of endothelial cells were significantly increased at days 4-5 and pericyte in days 4-7.MTT values reached 2.18 after adding VEGF, 2.99 after adding bFGF and 2.94 after adding both VEGF and bFGF as compared to 1.01 in the control group.ConclusionHypoxia stimulates proliferations of endothelial cell and pericyte.Both bFGF and VEGF stimulate proliferation of endothelial cell,however the effct of bFGF is shown to be a stronger.