高同型半胱氨酸血症大鼠冠状动脉内皮细胞单核细胞趋化蛋白1的表达

目的观察高同型半胱氨酸血症对大鼠冠状动脉内皮细胞表达单核细胞趋化蛋白1的影响,以明了冠状动脉粥样硬化性心脏病的发病机制。方法24只大鼠随机分成正常饮食对照组、高蛋氨酸饮食组、高蛋氨酸 叶酸饮食组、高半胱氨酸饮食组。每组6只,分别给予普通饲料;普通饲料加1.7%蛋氨酸;普通饲料加1.7%蛋氨酸和0.006%叶酸;普通饲料加1.2%半胱氨酸。饲养6周,采用高效液相色谱荧光检测法测定血浆总同型半胱氨酸浓度,免疫组织化学染色法检测大鼠冠状动脉左主干和左前降支内皮细胞单核细胞趋化蛋白1的表达。结果喂以高蛋氨酸饲料6周,可诱导大鼠高同型半胱氨酸血症。与正常饮食对照组比较,高蛋氨酸饮食组大鼠血浆总同型半胱氨酸浓度显著升高(P<0.01),冠状动脉内皮单核细胞趋化蛋白1的表达水平明显增强;高蛋氨酸 叶酸饮食组大鼠血浆总同型半胱氨酸水平较高蛋氨酸饮食组显著降低(P<0.01),其冠状动脉内皮单核细胞趋化蛋白1的表达水平也降低;高半胱氨酸饮食组大鼠血浆总同型半胱氨酸浓度,以及冠状动脉内皮单核细胞趋化蛋白1的表达水平与正常饮食对照组比较差异无显著性。结论高同型半胱氨酸血症促进了大鼠冠状动脉内皮细胞表达单核细胞趋化蛋白1,在冠状动脉粥样硬化性心脏病的发生和发展中起着重要的作用。

The Effect of Hyperhomocysteinemia on Monocyte Chemoattractant Protein-1 Expression in Rat Coronary Artery Endothelial Cells

Aim To investigate the effect of hyperhomocysteinemia on the expression of monocyte chemoattractant protein-1 in coronary endothelial artery cells in rats with hyperhomocysteinemia induced by excess methionine ingestion. Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups (n=6 for each group): Control group,High methionine group,High methionine folate group and High cysteine group.They were fed with normal diet,normal diet enriched by 1.7% methionine,and normal diet plus 1.7% methionine and 0.006% folate,normal diet with 1.2% cysteine,respectively,for 6 weeks.The levels of total plasma homocysteine were measured by high performance liquid chromatography and the expression of monocyte chemoattractant protein-1 on coronary endothelial artery cells of rats was detected by immunohistochemistry. Results A high-methionine diet for 6 weeks induced hyperhomocysteinemia.The plasma homocysteine level was significantly higher in rats of High methionine group than in rats of Control group(P<0.01),and the expression of monocyte chemoattractant protein-1 in coronary endothelial artery cells was also stronger.During supplementation of folate,normalization of homocysteine levels was accompanied by a marked reduction of monocyte chemoattractant protein-1 expression in coronary endothelial artery cells in rats of High methionine folate group(P<0.01),however,there were no significant differences in the plasma homocysteine and the expression of monocyte chemoattractant protein-1 in coronary endothelial artery cells between the Control group and the High cysteine group. Conclusions Hyperhomocysteinemia may play an important role in atherogenesis of coronary artery via stimulation of monocyte chemoattractant protein-1 expression in the coronary endothelial artery cells.