| 急性髓系白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D mRNA的表达与意义 |
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| 引用本文： | 肖广芬,唐雪元,李昕,曾璨. 急性髓系白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D mRNA的表达与意义[J]. 中国实验血液学杂志, 2010, 18(1): 15-18 |
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| 作者姓名： | 肖广芬 唐雪元 李昕 曾璨 |
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| 作者单位： | 中南大学湘雅三医院血液科,湖南长沙,410013 |
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| 摘 要： | 本研究探讨糖基化磷脂酰肌醇特异性磷脂酶D(glycosylphosphatidilinoditol-specific phospholipase D,GPI-PLD)在急性髓系白血病病人骨髓单个核细胞中的表达及其意义。采用半定量RT-PCR和酶转化底物的百分率的方法检测急性髓系白血病初发组、难治复发组、缓解组与正常对照组骨髓单个核细胞GPI-PLD酶活性和mRNA表达,并比较它们之间的差异。结果显示:急性髓系白血病初治组骨髓单个核细胞GPI-PLD mRNA表达和GPI-PLD活性分别为1.86±0.32、(46.96±7.15)%;缓解组分别为1.26±0.29和(33.36±5.13)%;难治复发组分别为1.79±0.19和(44.31±7.22)%;而正常对照组分别为1.27±0.23和(35.38±5.15)%。初治组和难治复发组的GPI-PLD活性与mRNA表达均明显高于正常对照组,差异具有统计学意义(p0.01);而缓解组与正常对照组之间比较,差异无统计学意义(p0.05)。结论:GPI-PLD活性和mRNA表达水平变化一致;初治组和难治复发组急性髓系白血病病人骨髓单个核细胞GPI-PLD活性与mRNA表达水平明显高于正常组。GPI-PLD是否在白血病的发生、发展过程中发挥一定作用,有待进一步研究。
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| 关 键 词： | 急性髓系白血病 GPI-PLD 骨髓单个核细胞 |
Expression and Activity of Glycosylphosphatidilinoditol-Specific Phospholipase D mRNA in Bone Marrow Mononuclear Cells Isolated from Patient with Acute Myeloid Leukemia and Their Significance |
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| XIAO Guang-Fen,TANG Xue-Yuan,LI Xin,ZENG Can. Expression and Activity of Glycosylphosphatidilinoditol-Specific Phospholipase D mRNA in Bone Marrow Mononuclear Cells Isolated from Patient with Acute Myeloid Leukemia and Their Significance[J]. Journal of experimental hematology, 2010, 18(1): 15-18 |
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| Authors: | XIAO Guang-Fen TANG Xue-Yuan LI Xin ZENG Can |
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| Affiliation: | (Department of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China ) |
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| Abstract: | This study was purposed to investigate the expression and significance of glycosylphosphatidilinoditol-specific phospholipase D(GPI-PLD) in bone marrow mononuclear cells(BMMNC) isolated from patients with acute myeloid leukemia(AML),GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase(PLAP) as a substrate and Triton X-114 phase partitioning.The GPI-PLD mRNA expression was measured by semi-quantitive reverse trans... |
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| Keywords: | AML GPI-PLD BMMNC |
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