Opiate binding to brain slices and ontogenesis of hypothalamic [3H]naloxone binding sites |
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Authors: | R Bhanot M Wilkinson |
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Affiliation: | Departments of Physiology and Biophysics and of Obstetrics and Gynaecology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7 |
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Abstract: | We have developed a radioligand binding assay based on the use of hypothalamic slices and have examined the ontogenesis of [3H]naloxone binding sites in male and female rats. [3H]NAL binding is reversible, saturable, stereospecific, of high affinity, readily displaceable by morphine and is sensitive to phenoxybenzamine. These characteristics suggest that [3H]NAL readily binds to opiate receptors in brain slices. With this assay we have demonstrated that: (a) there is an age-related increase in opiate binding sites in rat hypothalamus, (b) there are sex differences in the binding affinity of the sites and (c) the values of Bmax are approximately 2–5-fold higher than the levels previously reported from assays with brain homogenates. |
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Keywords: | Hypothalamus Opiate receptors Brain slices |
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