消炎痛抑制白色念珠菌菌丝生长的小鼠模型试验

目的:探讨消炎痛对白色念珠菌性阴道炎的治疗作用。方法:建立小鼠白色念珠菌阴道炎动物模型后,连续7天小鼠阴道内注入2 mmol/L的消炎痛100μl后,进行灌洗液涂片镜检,阴道灌洗液真菌荷载量菌落计数及阴道组织病理学检查。结果:消炎痛组仅见少量炎性细胞浸润(HE染色),阴道粘膜未见菌丝,仅见少量孢子(PAS染色);阴道灌洗液真菌载量计数有较低水平的CFU计数;灌洗液涂片镜检仅见少量孢子,未见菌丝。结论:消炎痛能抑制体内白色念珠菌菌丝生长和孢子增殖生长,对模型小鼠白色念珠菌性阴道炎具有显著的治疗作用。

Mouse model test of Indomethacin inhibiting mycelial growth of Candida albicans

Objective:To investigate the therapeutical efficacy of indomethacin on the candidiasis.Methods:Construct a murine model of vaginal candidiasis,then continuous infunding 100 μl indomethacin of 2mmol/L for seven days,then observing the douche smear with the microscope,counting colony count of mycetes capacity value in vagina douche and histopathology detection of vaginal tissue.Results:In indomethacin group there are only a few inflammatory cells(HE dyeing),little hypha,a few spores in vaginal mucosa(PAS dyeing),a low mycetes counting Colony in vagina douche and little hypha,a few spores in the douche smear.Conclusion:Indomethacin can inhibit mycelial growth and Sporogony of candida albicans in Mouse model,and has strikingly therapeutical efficacy on the candidiasis of the mouse.