Über poly[1-N-(4-isothiocyanatophenyl)carbamoyloxy-methyläthylen-co-1-hydroxymethyläthylen]e als träger zur immobilisierung von papain

Poly[1-N-(4-isothiocyanatophenyl)carbamoyloxymethylethylene-co-1-hydroxymethylethylene]s ( 5a – c ) were obtained by reaction of poly(allyl alcohol) ( 2 ) with 4-isothiocyanatophenyl isocyanate ( 3 ). In some cases 1,4-phenylenediisocyanate ( 4 ) was used as crosslinking agent. These reactive carriers were found to be suitable to immobilize enzymes by covalent bonds. The binding abilities of these polymers 5a – e for butylamine, 3,4-dichloroaniline, peptone (from lactalbumin), and papain were investigated. The enzymatic activity, the pH-optimum, the apparent Michaelis-constant K_M(app), and the activity dependance on temperature were determined for the immobilized papain.