| 赤首乌和白首乌的HPLC指纹图谱鉴定研究 |
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| 引用本文： | 刘训红,陈斌,蔡宝昌,王玉玺.赤首乌和白首乌的HPLC指纹图谱鉴定研究[J].医学教育探索,2005(11):1704-1706. |
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| 作者姓名： | 刘训红 陈斌 蔡宝昌 王玉玺 |
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| 作者单位： | 南京中医药大学 江苏南京210029(刘训红,陈斌,蔡宝昌),南京生物工程与医药科技发展有限公司 江苏南京210036(王玉玺) |
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| 基金项目： | 江苏省高校自然科学研究计划项目(03KJA360092) |
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| 摘 要： | 目的建立赤、白首乌HPLC指纹图谱鉴定方法。方法用RP-HPLC(DAD)法,梯度洗脱,测定赤、白首乌、加工炮制品及其何首乌粉的指纹图谱,并作相似度比较分析。结果何首乌不同样品指纹图谱相似度较好,而何首乌与白首乌的指纹图谱具有明显区别,何首乌炮制前后、白首乌加工前后也有差异。结论HPLC指纹图谱具有重现性好、特征性强、方法简便等特点,可用于赤、白首乌药材的鉴别。
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| 关 键 词： | 何首乌 白首乌 指纹图谱 高效液相色谱法 |
Identification on Radix Polygoni Multiflori and Radix Cynanchi Auriculati by HPLC fingerprint |
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| LIU Xun-hong,CHEN Bin,CAI Bao-chang,WANG Yu-xi.Identification on Radix Polygoni Multiflori and Radix Cynanchi Auriculati by HPLC fingerprint[J].Researches in Medical Education,2005(11):1704-1706. |
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| Authors: | LIU Xun-hong CHEN Bin CAI Bao-chang WANG Yu-xi |
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| Institution: | 1. Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; 2. Nanjing Bio-engineering and Medical Technology Development Co. , Ltd. , Nanjing 210036, China |
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| Abstract: | Objective To establish HPLC fingerprint for the identification of Radix Polygoni Multiflori and Radix Cynanchi Auriculati. Methods Chromatographic fingerprint of Radix Polygoni Multiflori, Radix Cynanchi Auriculati, Radix Polygoni Multiflori Preparata, and "HESHOU WU FEN" was determined by RP-HPLC (DAD) and the gradient elution mode applied in chromatographic separation, data were analysed by Fingerprint Similarity Evaluation Software to compare the similarity of samples. Results Radix Polygoni Multiflori from different samples were of high similarity, but Radix Polygoni Multiflori and Radix Cynanchi Auriculati showed evident difference in fingerprint, and there was difference in fingerprint through processing them. Conclusion HPLC fingerprint method is repeatable and feasible and can be used for the identification of Radix Polygoni Multiflori and Radix Cynanchi Auriculati. |
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| Keywords: | Radix Polygoni Multiflori Radix Cynanchi Auriculati fingerprint HPLC |
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