西替利嗪对皮肤细胞炎症中单核趋化蛋白-1表达的干预作用

目的考察西替利嗪(CET)对皮肤细胞炎症模型中单核趋化蛋白-1(MCP-1)表达的干预作用。方法组胺(HIS)和IFN-γ刺激真皮成纤维细胞(DF)和人角质形成细胞株HaCaT细胞，采用RT-PCR和ELISA法考察两种细胞MCP-1 mRNA和蛋白表达水平。结果与对照组比较，HIS(10 μmol·L^-1)和IFN-γ(20 ng·mL^-1)组显著上调两种细胞(DF和HaCaT) MCP-1 mRNA表达，同时分别使DF的MCP-1蛋白分泌量增加3.5倍和8.4倍，对HaCaT细胞也有相似的影响趋势； CET (1和10 μmol·L^-1) 显著地抑制了它们对细胞MCP-1蛋白产生的增强作用。结论CET可能通过抑制MCP-1的表达而发挥抗皮肤炎症作用。

Intervention of cetirizine on monocyte chemoattractant protein-1 in cutaneous inflammation

AIM: To study the intervention of cetirizine on monocyte chemoattractant protein-1 (MCP-1) in different cutaneous inflammation models. METHODS: Histamine and IFN-gamma stimulated dermal fibroblast cells and HaCaT cells to mimic cutaneous inflammation. Expression of MCP-1 was assessed by means of RT-PCR and ELISA. RESULTS: Compared with the control group of dermal fibroblast (DF) cells and HaCaT cells, MCP-1 mRNA was significantly upregulated by histamine (10 micromol x L(-1)) and IFN-gamma (20 ng x mL(-1)). The protein secretions of MCP-1 were increased 3.5 fold and 8.4 fold in DF cells, respectively. The similar tendency was observed in HaCaT cells. The enhancing effects of histamine and IFN-gamma on MCP-1 protein production were significantly inhibited by cetirizine (1 and 10 micromol x L(-1)) in DF and HaCaT cells. CONCLUSION: Cetirizine may exert the anti-inflammatory effect of skin via inhibiting MCP-1 expression.