| 大鼠全脑缺血再灌注后HAX-1蛋白与皮质神经元凋亡的关系 |
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| 引用本文： | 范沁林,焦岩,刘国军,周振华,张波,陈林,甘立霞,陈康宁. 大鼠全脑缺血再灌注后HAX-1蛋白与皮质神经元凋亡的关系[J]. 第三军医大学学报, 2010, 32(6): 584-587 |
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| 作者姓名： | 范沁林 焦岩 刘国军 周振华 张波 陈林 甘立霞 陈康宁 |
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| 作者单位： | 第三军医大学:西南医院神经内科,重庆,400038;第三军医大学:基础医学部生物化学与分子生物学教研室,重庆,400038 |
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| 摘 要： | 目的探讨大鼠全脑缺血再灌注后皮质组织中HAX-1蛋白(HS1-associated protein X-1,HAX-1)与皮质神经元凋亡的关系。方法建立大鼠四血管法全脑缺血模型,将实验动物分为5组(n=5):正常组,缺血6、24、48、72h组。采用HE染色,观察大鼠皮层组织病理变化;采用免疫组化、TUNEL法检测大鼠全脑缺血再灌注后HAX-1蛋白的表达以及皮质神经元凋亡蛋白Caspase-3的表达情况。结果HAX-1蛋白在缺血后6h表达最高(37.60±3.45),24、48、72h降低[分别为(11.40±1.14)、(10.40±1.52)、(9.80±1.30)],且低于正常水平(P<0.01);Caspase-3蛋白随着缺血时间的延长逐渐增高[6、24、48、72h分别为(72.80±5.49)、(106.20±6.91)、(129.00±19.74)、(166.20±15.32)](P<0.01),并伴随神经元凋亡的数目逐渐增加[(92.00±9.06)、(133.80±10.64)、(157.00±10.83)、(187.80±14.96)]。结论全脑缺血再灌注后皮质神经元中HAX-1蛋白在短期...
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| 关 键 词： | 大鼠 全脑缺血再灌注 HAX-1 Caspase-3 神经元凋亡 |
Relationship between HAX-1 expression and neuron apoptosis after cerebral ischemia reperfusion injury in rats |
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| Fan Qinlin,Jiao Yan,Liu Guojun,Zhou Zhenhua,Zhang Bo,Chen Lin,Gan Lixia,Chen Kangning. Relationship between HAX-1 expression and neuron apoptosis after cerebral ischemia reperfusion injury in rats[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(6): 584-587 |
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| Authors: | Fan Qinlin Jiao Yan Liu Guojun Zhou Zhenhua Zhang Bo Chen Lin Gan Lixia Chen Kangning |
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| Affiliation: | 1Department of Neurology;Southwest Hospital;2Department of Biochemistry and Molecular Biology;College of Basic Medical Sciences;Third Military Medical University;Chongqing;400038;China |
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| Abstract: | Objective To investigate the relationship between HS1-associated protein X-1 (HAX-1) expression and neuron apoptosis in rats after global cerebral ischemia reperfusion injury. Methods Rats were subjected to global cerebral ischemia reperfusion injury through four-vessel occlusion, and then the experimental rats were divided into 5 groups, normal group (NC), 6, 24, 48 and 72 h post injury groups (n=5 for each group). Immunohistochemistry and TUNEL were used to detect HAX-1 expression and activated Caspase-3,... |
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| Keywords: | rats ischemia reperfusion HS1-associated protein X-1 Caspase-3 neuron apoptosis |
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