p53、bcl-2基因在喉鳞癌中的蛋白表达及其临床意义

目的　探讨细胞凋亡相关基因 p5 3、bcl- 2与喉鳞癌的关系。方法　应用免疫组化 ABC法对 6 0例喉鳞癌和 8例声带息肉标本中的 p5 3、bcl- 2基因的蛋白表达进行检测。结果　 6 0例喉鳞癌中 p5 3、bcl- 2基因蛋白的表达阳性率均较高 ,分别为 6 1.7% (37/6 0 )和 43.3% (2 6 /6 0 ) ,并且其阳性表达率与喉鳞癌的病理分化程度和颈淋巴结转移有关 (P<0 .0 5 ) ,而与喉鳞癌的临床分期和分型无关 (P>0 .0 5 )。8例声带息肉标本的 p5 3、bcl- 2基因蛋白的表达均为阴性。结论　本实验从蛋白水平上证实了喉鳞癌与 p5 3、bcl- 2基因的关系 ,提示此两种基因可能参与了喉鳞癌细胞凋亡的调节 ,对喉鳞癌的发生可能起着重要的作用。

The Protein Expression of p53 and bcl-2 in Laryngeal Squamous Cell Carcinoma

Objective To detect the relationship of p53 and bcl 2 gene to laryngeal carcinoma. Methods p53 and bcl 2 protein expressions were examined in the specimens of 60 laryngeal carcinomas and 8 vocal cord polyps by immunohistochemistry method. Results Overexpressions of p53 and bcl 2 were found in the laryngeal carcinoma specimens; the positive rates of p53 and bcl 2 protein expression were 61.7%(37/60) and 43.3%(26/60), respectively. Nagative results were observed in all the vocal cord polyp specimens. The positive rates of p53 and bcl 2 expression were related to the histological grade and cervical lymphatic metastasis of laryngeal carcinoma but not related to clinical stage and tumor types. Conclusion The results of this study support the hypothesis that p53 and bcl 2 gene may be involved in the apoptosis of laryngeal carcinoma and they may play an important role in the pathogenesis of laryngeal cancer.