| 敌百虫经ERK1/2通路影响类固醇激素的合成 |
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| 引用本文： | 瞿建华,江俊康,陈刚. 敌百虫经ERK1/2通路影响类固醇激素的合成[J]. 毒理学杂志, 2010, 0(2) |
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| 作者姓名： | 瞿建华 江俊康 陈刚 |
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| 作者单位： | 南通大学公共卫生学院职业卫生与环境毒理学教研室; |
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| 基金项目： | 国家自然科学基金(20777042); 南通市应用研究计划项目(K2007020) |
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| 摘 要： | 目的初步探讨细胞外信号调节激酶(ERK1/2)通路在敌百虫抑制小鼠睾丸间质细胞瘤细胞(MLTC-1)类固醇激素合成中的作用。方法运用放射免疫法检测不同浓度敌百虫及加入ERK1/2通路抑制剂UO126后对MLTC-1细胞孕酮合成的影响;采用蛋白质印迹技术(Western-blot)检测敌百虫对ERK1/2磷酸化表达的影响。结果敌百虫显著抑制MLTC-1细胞孕酮的合成,并且随敌百虫剂量的增加,孕酮合成量呈下降趋势;当同时加入UO126后,随敌百虫染毒剂量的升高孕酮合成量的下降趋势更为明显;敌百虫与UO126一样能明显抑制ERK1/2的磷酸化,而对总ERK1/2表达无明显影响。结论在本试验条件下,敌百虫能明显抑制MLTC-1细胞的孕酮合成,其机制可能是通过ERK1/2通路。
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| 关 键 词： | 敌百虫 ERK1/2通路 类固醇激素合成 |
Trichlorfon inhibits steroidogenesis expression via ERK1 /2 signal pathway |
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| QU Jian-hua,JIANG Jun-kang,CHEN Gang. Trichlorfon inhibits steroidogenesis expression via ERK1 /2 signal pathway[J]. Journal of Toxicology, 2010, 0(2) |
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| Authors: | QU Jian-hua JIANG Jun-kang CHEN Gang |
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| Affiliation: | QU Jian-hua,JIANG Jun-kang,CHEN Gang (Department of Occupational Health , Enviromental Toxicology,School of Public Health,Nantong University,Nantong Jiangsu 226007,China) |
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| Abstract: | Objective To investigate the role of extracellular signal regulated kinase (ERK1/2) signal pathway in the inhibition of steroidogenesis of MLTC-1 cells by trichlorfon. Methods After treated with different concentrations of trichlorfon with or without UO126,the progesterone secretion of MLTC-1 cells was detected by RIA. Western blot analysis was used to assess the expression of ERK1 /2 phosphorylation. Results The progesterone production of MLTC-1 cells was decreased in a dose-effect manner depending on tric... |
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| Keywords: | Trichlorfon ERK1/2 signal pathway Steroidogenesis |
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