| 肌氨酸氧化酶法和碱性苦味酸速率法检测血清肌酐结果比较和偏倚评估 |
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| 引用本文： | 黄霞梅,伍惠玲,钟慧芝,李海炜,秦雪,李山. 肌氨酸氧化酶法和碱性苦味酸速率法检测血清肌酐结果比较和偏倚评估[J]. 重庆医学, 2011, 40(33): 3372-3374,3434. DOI: 10.3969/j.issn.1671-8348.2011.33.020 |
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| 作者姓名： | 黄霞梅 伍惠玲 钟慧芝 李海炜 秦雪 李山 |
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| 作者单位： | 1. 广西医科大学第一附属医院临床医学实验部,南宁,530021
2. 广西医科大学医学检验系,南宁,530021 |
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| 基金项目： | 广西卫生厅科研课题(z2008158) |
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| 摘 要： | 目的比较肌氨酸氧化酶法和碱性苦味酸速率法测定血清肌酐的结果,并探讨胆红素对两种测定方法的影响。方法同时用两种方法检测非黄疸标本和黄疸标本,对测定结果用Excel2003和SPSS13.0软件进行处理。结果两种方法检测血清肌酐结果高度相关,有很好的可比性(P<0.05),但碱性苦味酸速率法的测定结果比肌氨酸氧化酶法高。两种方法测定肌酐结果有恒定偏倚,且预期偏倚随肌酐浓度的降低而增大。用黄疸患者血清标本进行比较,肌氨酸氧化酶法和碱性苦味酸速率法测定结果仍具有很好的相关性(P<0.05)。用粉剂总胆红素标准品作为干扰物发现胆红素对两种方法检测血清肌酐值均有正干扰,而换Beckman总胆红素标准液则得出结果为两种方法均不受胆红素的影响。结论两种方法检测非黄疸血清肌酐结果具有很好的可比性,因其方法原理和仪器不同,肌酐测定需建立不同参考值范围。
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| 关 键 词： | 肌氨酸氧化酶 碱性苦味酸速率法 血清肌酐 比对 |
Comparision and bias analysis of enzymatic and jaffe kinetic methods for the serum creatinine determination |
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| Huang Xiamei,Wu Huiling,Zhong Huizhi,Li Haiwei,Qin Xue,Li Shan. Comparision and bias analysis of enzymatic and jaffe kinetic methods for the serum creatinine determination[J]. Chongqing Medical Journal, 2011, 40(33): 3372-3374,3434. DOI: 10.3969/j.issn.1671-8348.2011.33.020 |
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| Authors: | Huang Xiamei Wu Huiling Zhong Huizhi Li Haiwei Qin Xue Li Shan |
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| Affiliation: | Huang Xiamei1,Wu Huiling1,Zhong Huizhi1,Li Haiwei1,Qin Xue1,Li Shan2 (1.Department of Laboratory Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China,2.The Faculty of Laboratory Medicine,Guangxi Medical University,China) |
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| Abstract: | Objective To compare the results of serum creatinine detected by both Enzymatic and Jaffe kinetic methods,and study the impact of jaundice serum on two determination methods.Methods Non-jaundice serum and jaundice serum were detected respectively by both Enzymatic assay and Jaffe kinetic assay with automatic biochemical analyzer Hitachi 7600 and Beckman Coulter LX20.The results were analyzed by SPSS13.0 and Excel2003.Results The results of the serum creatinine detected by the two methods were highly relevan... |
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| Keywords: | sarcosine oxidase jaffe kinetic assay creatinine method comparison |
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