| 复方扶正中药含药血清对急性髓细胞白血病患者骨髓单个核细胞来源树突状细胞影响 |
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| 引用本文： | 黄礼明,胡展瑞,华昭,王雷,孙晶晶. 复方扶正中药含药血清对急性髓细胞白血病患者骨髓单个核细胞来源树突状细胞影响[J]. 中华中医药杂志, 2011, 0(11): 2707-2710 |
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| 作者姓名： | 黄礼明 胡展瑞 华昭 王雷 孙晶晶 |
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| 作者单位： | 贵阳中医学院第二附属医院血液科;广西梧州市中医院血液肿瘤科;贵州省清镇市疾病控制中心流行病科;贵阳中医学院; |
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| 摘 要： | 目的:研究复方扶正中药对急性髓细胞白血病完全缓解期(AML-CR)患者骨髓单个核细胞(BMNC)来源树突状细胞(DC)的影响。方法:分离培养AML-CR患者的BMNC,用不同浓度复方扶正中药含药血清与之进行体外培养,倒置显微镜下观察诱导转化的DC形态学特征,流式细胞术检测DC表面分子CD86、CD-1a、HLA-DR的表达。以诱导转化的DC分别与异体T细胞、自体T细胞共同培养,以激发T细胞,MTT法检测激发后的T细胞在不同效靶比时对人白血病细胞株K562的杀伤作用。结果:复方扶正中药含药血清与AML-CR患者BMNC培养能促进BMNC分化为形态特征典型的DC,高表达成熟DC的特异性标记(CD86、CD-1a和HLA-DR),并激发T细胞杀伤K562细胞。结论:复方扶正中药对AML-CR患者BMNC来源DC具有明显的促进作用。
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| 关 键 词： | 急性髓细胞白血病 中药 树突细胞 |
Effect of the serum with compound of Fu Zheng TCM to the induction of DC of AML-CR patients with source of BMNC |
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| HUANG Li-ming,HU Zhan-rui,HUA Zhao,WANG Lei,SUN Jing-jing. Effect of the serum with compound of Fu Zheng TCM to the induction of DC of AML-CR patients with source of BMNC[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2011, 0(11): 2707-2710 |
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| Authors: | HUANG Li-ming HU Zhan-rui HUA Zhao WANG Lei SUN Jing-jing |
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| Affiliation: | HUANG Li-ming1,HU Zhan-rui2,HUA Zhao3,WANG Lei4,SUN Jing-jing4(1Hematology Department,the Second Affiliated Hospital of Guiyang College of TCM,Guiyang 550003,China,2Hematology and Oncology Department,TCM Hospital of Wuzhou City,Wuzhou 543000,3Epidemiology Department,CDC of Qingzhen,Guizhou Province,Qingzhen 551400,4Guiyang College of TCM,Guiyang 550002,China) |
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| Abstract: | Objective: Research on the effect of TCM compound Fu Zheng to the derived dendritic cells(dendritic cells,DC) of patients with bone marrow mononuclear cells(BMNC) during the time of acute myeloid leukemia complete remission(AML-CR).Methods: Isolate BMNC from AML-CR patients,and culture it combined with serums of different concentrations of compound Chinese herb medicine.Therefore,transformation was observed under inverted microscope,the morphological characteristics of DC,DC surface molecules by flow cytome... |
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| Keywords: | Acute myeloid leukemia Chinese medicine Dendrite cell |
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