| 人参皂甙对内毒素所致大鼠急性肺损伤的影响 |
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| 引用本文： | 巩秀丽,毛毅敏,孙瑜霞,刘艳,张咏梅,潘丽娟.人参皂甙对内毒素所致大鼠急性肺损伤的影响[J].中华中医药杂志,2011(11):2743-2745. |
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| 作者姓名： | 巩秀丽 毛毅敏 孙瑜霞 刘艳 张咏梅 潘丽娟 |
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| 作者单位： | 河南科技大学第一附属医院; |
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| 基金项目： | 河南省科技攻关课题(No.072102330029)~~ |
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| 摘 要： | 目的:探讨人参皂甙对内毒素所致大鼠急性肺损伤的保护作用及作用机制。方法:将成年大鼠30只按雌雄对半分为3组(每组10只):正常对照组(A组)、内毒素组(B组)、人参皂甙组(C组)。股静脉注射LPS(5mg/kg)复制大鼠ALI模型,比较各组大鼠的一般状况,观测各组大鼠肺脏的大体形态,测定动脉血氧分压(PaO2),氧合指数(PaO2/FiO2),肺湿重/干重(W/D),做病理切片观察肺组织学改变及免疫组织化学法检测肺组织中SP-A蛋白水平的表达情况。结果:与A组比较,B组的PaO2,PaO2/FiO2显著降低(P<0.01),W/D显著增高(P<0.01);C组的PaO2,PaO2/FiO2相对降低(P<0.05),W/D相对增高(P<0.05),B组和C组间PaO2,PaO2/FiO2和W/D存在显著差异(P<0.01)。免疫组化半定量结果显示,A组大鼠肺组织中SP-A蛋白呈强阳性表达。B组与A组相比,明显减弱(P<0.01)。C组与B组相比,B组大鼠肺组织中SP-A蛋白明显增强,呈阳性(P<0.01)。结论:人参皂甙可能通过上调SP-A蛋白的表达保护急性肺损伤。
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| 关 键 词： | 急性肺损伤 人参皂甙 内毒素 肺表面活性蛋白A |
Effection of Ginseng saponins on acute lung injury in rats by endotoxin |
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| GONG Xiu-li,MAO Yi-min,SUN Yu-xia,LIU Yan,ZHANG Yong-mei,PAN Li-juan.Effection of Ginseng saponins on acute lung injury in rats by endotoxin[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(11):2743-2745. |
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| Authors: | GONG Xiu-li MAO Yi-min SUN Yu-xia LIU Yan ZHANG Yong-mei PAN Li-juan |
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| Institution: | GONG Xiu-li,MAO Yi-min,SUN Yu-xia,LIU Yan,ZHANG Yong-mei,PAN Li-juan(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,China) |
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| Abstract: | Objective: In this experiment,we can explore the protection function and mechanism from ginseng saponins on acute lung injury in rats by endotoxin.Methods: Thirty adult rats are deveided into three groups(n=10): controlgroup(A),lipopolysaccharide group(B),the intervention group from ginsenosides(C).Duplicating the model of acute lung injury in rats by injecting endotoxin(5mg/kg) from femoral vein,we can compare the general state of the rats and observe form of the lung.Determine the partial pressure of oxyg... |
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| Keywords: | Acute lung injury Ginsenoside saponins lipopolysaccharide Endotoxin SP-A |
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