| HPLC法测定银黄喷雾剂中麝香草酚及香荆芥酚的含量 |
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| 引用本文： | 黄家卫,盛振华,张蓓,吴巧凤.HPLC法测定银黄喷雾剂中麝香草酚及香荆芥酚的含量[J].中华中医药杂志,2011(11):2693-2695. |
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| 作者姓名： | 黄家卫 盛振华 张蓓 吴巧凤 |
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| 作者单位： | 浙江中医药大学; |
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| 基金项目： | 浙江省教育厅科研项目(No.20071129); 浙江省分析测试科技计划项目(No.2009F70024)~~ |
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| 摘 要： | 目的:建立HPLC法测定银黄喷雾剂中麝香草酚及香荆芥酚的含量。方法:色谱柱为Hypersil BDS C18(250mm×4.6mm,5μm),以甲醇-2%醋酸(60:40)为流动相,流速1.0mL/min,检测波长为274nm。结果:麝香草酚的线性范围为0.02-1.43μg(r=0.9997),平均回收率为99.43%,RSD为2.01%,香荆芥酚的线性范围为0.027-0.870μg(r=0.9999),平均回收率为99.76%,RSD为1.73%。结论:该方法简单、快速、准确,可为银黄喷雾剂的质量控制提供实验依据。
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| 关 键 词： | 高效液相色谱 银黄喷雾剂 麝香草酚 香荆芥酚 |
Determine the contents of Thymol and Carvacrol from Yinhuang Spray by HPLC |
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| HUANG Jia-wei,SHENG Zhen-hua,ZHANG Bei,WU Qiao-feng.Determine the contents of Thymol and Carvacrol from Yinhuang Spray by HPLC[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(11):2693-2695. |
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| Authors: | HUANG Jia-wei SHENG Zhen-hua ZHANG Bei WU Qiao-feng |
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| Institution: | HUANG Jia-wei,SHENG Zhen-hua,ZHANG Bei,WU Qiao-feng(Zhejiang Chinese Medical University,Hangzhou 310053,China) |
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| Abstract: | Objective: To establish a HPLC method for the thymol and carvacrol contents determination of Yinhuang Spray.Methods: The column was Hypersil BDS C18(250mm × 4.6mm,5μm),methanol-2% acetic acid(60:40) as mobile phase,flow rate of 1.0mL/min,detection wavelength 274nm.Results: The linear range of thymol 0.02-1.43μg(r=0.9997),the average recovery was 99.43%,RSD was 2.01%(n=9),carvacrol linear range 0.027-0.870μg(r=0.9999),the average recovery was 99.76%,RSD was 1.73%(n=9).Conclusion: This method is simple,fast,and accurate which can provide experimental basis for the quality control of Yinhuang Spray. |
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| Keywords: | HPLC Yinhuang Spray Thymol Carvacrol |
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