| 加味小青龙汤对内毒素致急性损伤大鼠肺组织TLR4 mRNA表达的影响 |
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| 引用本文： | 杨爱东,李文雯,王利霞,汪东颖,吴中华,苏中昊,庞慧芳. 加味小青龙汤对内毒素致急性损伤大鼠肺组织TLR4 mRNA表达的影响[J]. 中华中医药杂志, 2011, 0(12): 2997-3000 |
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| 作者姓名： | 杨爱东 李文雯 王利霞 汪东颖 吴中华 苏中昊 庞慧芳 |
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| 作者单位： | 上海中医药大学基础医学院;上海中医药大学科技实验中心; |
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| 基金项目： | 国家中医药管理局重点学科项目; 上海市重点学科建设项目(No.S30301); 上海市教育委员会科研创新项目(No.11YZ67); 上海中医药大学名师研究室项目(No.Z1008210); 上海高校选拔培养优秀青年教师科研专项基金(No.P13908)~~ |
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| 摘 要： | 目的:探讨加味小青龙汤对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠肺组织Toll样受体4(TLR4)mRNA表达的影响。方法:将72只雄性Wistar大鼠随机分为:对照组、模型组、地塞米松组和加味小青龙汤(小、中、大剂量)组,每组再分为4h和8h两个亚组,每个亚组6只。尾静脉注射LPS 10mg/kg制备大鼠ALI模型。地塞米松组和加味小青龙汤(小、中、大剂量)组分别于造模前灌胃地塞米松和加味小青龙汤,2次/d,3d。采用实时荧光定量聚合酶链反应(PCR)检测TLR4 mRNA的表达,并于光镜下观察大鼠肺组织病理变化。结果:与模型组相比,在8h组中,加味小青龙汤(小、大剂量)组的TLR4 mRNA表达均明显降低(P<0.01)。病理学观察,模型组大鼠肺组织出现大片出血及坏死,各治疗组大鼠肺组织病理改变明显轻于模型组,肺细支气管偶见炎症改变,水肿较轻,中性粒细胞(PMN)和红细胞渗出较少。结论:加味小青龙汤能减轻内毒素致ALI大鼠肺组织损伤,对肺损伤有保护作用,其机制可能与其能降低内毒素致ALI大鼠肺组织TLR4 mRNA的表达有关。
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| 关 键 词： | 加味小青龙汤 急性肺损伤 Toll样受体-4 |
Effects of Jiawei Xiaoqinglong Decoction on expressions of lung TLR4 mRNA in the rats with acute lung injury caused by lipopolysaccharide |
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| YANG Ai-dong,LI Wen-wen,WANG Li-xia,WANG Dong-ying,WU Zhong-hua,SU Zhong-hao,PANG Hui-fang. Effects of Jiawei Xiaoqinglong Decoction on expressions of lung TLR4 mRNA in the rats with acute lung injury caused by lipopolysaccharide[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2011, 0(12): 2997-3000 |
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| Authors: | YANG Ai-dong LI Wen-wen WANG Li-xia WANG Dong-ying WU Zhong-hua SU Zhong-hao PANG Hui-fang |
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| Affiliation: | YANG Ai-dong1,LI Wen-wen1,WANG Li-xia1,WANG Dong-ying1,WU Zhong-hua2,SU Zhong-hao1,PANG Hui-fang1 (1College of Basic Medical Sciences,Shanghai University of TCM,Shanghai 201203,China,2Technology Laboratory Center,China) |
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| Abstract: | Objective: To investigate the effects of Jiawei Xiaoqinglong Decoction on lung of expressions of TLR4mRNA in the rats with acute lung injury caused by lipopolysaccharide(LPS).Methods: Seventy-two male Wistar rats were randomly divided into six groups including normal control,LPS model,dexamethasone and Jiawei Xiaoqinglong Decoction(Small,medium and large doses) groups.Each group had two subgroups,4h and 8h after LPS were injected.Each subgroup had six rats.The ALI rat model was established by LPS through in... |
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| Keywords: | Jiawei Xiaoqinglong Decoction Acute lung injury TLR4 |
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