| 微型光谱仪检测血清二氧化碳的研究 |
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| 引用本文： | 傅灵媛,吴杰红,张志伦,蒲晓允.微型光谱仪检测血清二氧化碳的研究[J].重庆医学,2011,40(33):3391-3392,3425. |
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| 作者姓名： | 傅灵媛 吴杰红 张志伦 蒲晓允 |
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| 作者单位： | 第三军医大学新桥医院检验科,重庆,400037 |
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| 基金项目： | 解放军总装备部预研基金(2007E056) |
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| 摘 要： | 目的研究一种基于微型光谱仪和干化学试剂技术快速检测血清二氧化碳浓度的方法。方法根据酶法制备干粉二氧化碳试剂,研究其在微型光谱仪上的检测方法,并对试剂及仪器的性能进行评价,进行统计学分析。结果制备的干试剂结构疏松、溶解性好,90d内反应稳定。用546nm波长检测,其线性范围为15～40mmol/L,批内变异系数(CV)<4%,批间CV<6%,回收率为98.8%～105.8%。当血清内胆红素(TB)<290.40μmol/L,三酰甘油(TG)<11.20mmol/L,无明显干扰,与全自动生化分析仪酶法结果的相关性良好(r=0.956)。结论采用微型光谱仪干试剂法测定血清二氧化碳,是一种可行的检测方法,可用于血清二氧化碳的快速批量测定。
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| 关 键 词： | 二氧化碳 微型光谱仪 干试剂 |
Experimental study on the detection of carbon dioxide based on dry chemistry method with micro spectrometer |
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| Fu Lingyuan,Wu Jiehong,Zhang Zhilun,Pu Xiaoyun.Experimental study on the detection of carbon dioxide based on dry chemistry method with micro spectrometer[J].Chongqing Medical Journal,2011,40(33):3391-3392,3425. |
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| Authors: | Fu Lingyuan Wu Jiehong Zhang Zhilun Pu Xiaoyun |
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| Institution: | Fu Lingyuan,Wu Jiehong,Zhang Zhilun,Pu Xiaoyun(Department of Laboratory Medicine,Xinqiao Hospital,the Third Military Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To establish a new method for rapid detection of carbon dioxide based on micro spectrometer and dry reagent.Methods The dry powder carbon dioxide reagent was prepared through enzymatic method,its detection method in the micro spectrometer was explored and the performance of both the reagent and the equipment was evaluated with statistical analysis.Results The prepared dry reagents were of rarefaction structures and with good reaction solubility for 90 days after being lyophilized.The linear range ... |
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| Keywords: | carbon dioxide micro spectrometer dry reagent |
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