| 基因修饰的树突状细胞体外免疫功能的初步研究 |
| |
| 引用本文： | 孔练花,李军,韩亚萍,刘源,黄祖瑚.基因修饰的树突状细胞体外免疫功能的初步研究[J].东南国防医药,2011,13(6):481-484. |
| |
| 作者姓名： | 孔练花 李军 韩亚萍 刘源 黄祖瑚 |
| |
| 作者单位： | 南京医科大学第一附属医院感染病科,江苏南京,210029 |
| |
| 基金项目： | 国家自然科学基金资助项目(30972618) |
| |
| 摘 要： | 目的 制备转染乙型肝炎病毒核心抗原(HBcAg)基因、人外周血单个核细胞(PBMC)来源的树突状细胞(DC)疫苗,观察HBcAg的表达效率,检测其在体外诱导的细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTL)对HepG2.2.15细胞的杀伤效应.方法 体外诱导并培养DC;将HBcAg基因导入...
|
| 关 键 词： | 树突状细胞 基因转染 脂质体 T淋巴细胞 细胞毒性 |
The preliminary study on the immune function of the gene modified dendritic cells in vitro |
| |
| KONG Lian-hua,LI Jun,HAN Ya-ping,LIU Yuan,HUANG Zu-hu.The preliminary study on the immune function of the gene modified dendritic cells in vitro[J].Journal of Southeast China National Defence Medical Science,2011,13(6):481-484. |
| |
| Authors: | KONG Lian-hua LI Jun HAN Ya-ping LIU Yuan HUANG Zu-hu |
| |
| Institution: | KONG Lian-hua,LI Jun,HAN Ya-ping,LIU Yuan,HUANG Zu-hu.Department of Infection Disease,First Affiliated Hospital,Nanjing Medical University,Nanjing,Jiangsu 210029,China |
| |
| Abstract: | Objective To prepare the dendritic cell(DC) vaccine derived from peripheral blood monouclear cells(PBMC) and transfected by the cation liposome bearing the hepatitis B core gene in vitro,and to observe the inductive therapeutic effect of cytotoxic T lymphocyte(CTL) on HepG2.2.15 cells.Methods DC proliferated from human peripheral blood monocytes by adding GM-CSF and IL-4 and PBMC centrifugated by the HES centrifugation were cultured and stimulated into DC by granulocyte-macrophage colony stimulating factor(... |
| |
| Keywords: | dentritic cells gene transfection liposome T lymphocyte cytotoxicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
