| 黄芪糖蛋白、雷公藤甲素、雷帕霉素和氢化考的松体外免疫抑制作用的比较研究 |
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| 引用本文： | 杨向竹,赵俊云,崔向青,胡秀华,冯前进,刘亚明.黄芪糖蛋白、雷公藤甲素、雷帕霉素和氢化考的松体外免疫抑制作用的比较研究[J].中华中医药杂志,2011(10):2411-2414. |
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| 作者姓名： | 杨向竹 赵俊云 崔向青 胡秀华 冯前进 刘亚明 |
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| 作者单位： | 北京中医药大学基础医学院;山西中医学院方剂教研室; |
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| 基金项目： | 山西省自然科学基金资助项目(No.2009011013-1)~~ |
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| 摘 要： | 目的:比较黄芪糖蛋白与雷公藤甲素、雷帕霉素、氢化考的松的体外免疫抑制作用。方法:不同浓度黄芪糖蛋白、雷公藤甲素、雷帕霉素、氢化考的松作用于小鼠脾细胞,MTT法测定4种药物对ConA、LPS诱导的淋巴细胞增殖的影响,流式细胞仪测定4种药物对脾细胞培养上清液中细胞因子IFN-γ、IL-6含量的影响。结果:4种药物对体外培养的脾细胞增殖均表现出显著的抑制活性(P<0.05),药效强度依次为雷公藤甲素、雷帕霉素、氢化考的松、黄芪糖蛋白。但黄芪糖蛋白主要表现为对T细胞的抑制作用。黄芪糖蛋白促进淋巴细胞分泌IFN-γ、IL-6,而其它3种药物却抑制IFN-γ、IL-6的分泌(P<0.05)。结论:黄芪糖蛋白对于淋巴细胞体外增殖的抑制作用不同于雷公藤甲素、雷帕霉素、氢化考的松,推测其具有独特的作用机制。
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| 关 键 词： | 黄芪糖蛋白 免疫抑制剂 增殖 细胞因子 |
Comparative study of immunosuppression of HuangQi glycoprotein,triptolide,rapamycin and hydrocortisone in vitro |
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| YANG Xiang-zhu,ZHAO Jun-yun,CUI Xiang-qing,HU Xiu-hua,FENG Qian-jin,LIU Ya-ming.Comparative study of immunosuppression of HuangQi glycoprotein,triptolide,rapamycin and hydrocortisone in vitro[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(10):2411-2414. |
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| Authors: | YANG Xiang-zhu ZHAO Jun-yun CUI Xiang-qing HU Xiu-hua FENG Qian-jin LIU Ya-ming |
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| Institution: | YANG Xiang-zhu1,ZHAO Jun-yun1,CUI Xiang-qing1,HU Xiu-hua1,FENG Qian-jin2,LIU Ya-ming2(1School of Basic Medical Sciences,Beijing University of Chinese Medicine,Beijing 100029,China,2Depatment of Formulas of Chinese Medicine,Shanxi University of TCM,Taiyuan 030024,China) |
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| Abstract: | Objective: To compare the difference between immunosuppressants of HuangQi glycoprotein(HQGP),Triptolide(Tri),Rapamycin(RAPA) and Hydrocortisone(HC) in vitro.Methods: The effects of different immunosuppressants on the lymphocytes proliferation treated by mitogens were measured with MTT.Having been cultured for 48 hours,supernatants were collected and tested for cytokine production by flow cytometry.Results: HQGP could notably inhibit the proliferation of lymphocytes induced by mitogens in dose-dependent fas... |
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| Keywords: | HuangQi glycoprotein Immunosuppressant Proliferation Cytokine |
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