| 反相高效液相色谱法测定莪术油注射液中莪术醇和牛儿酮的含量 |
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| 引用本文： | 曹玲,万嵘,王玉.反相高效液相色谱法测定莪术油注射液中莪术醇和牛儿酮的含量[J].中国生化药物杂志,2007,28(6):394-396. |
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| 作者姓名： | 曹玲 万嵘 王玉 |
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| 作者单位： | 1. 江苏省药品检验所,江苏,南京,210008
2. 南京工业大学,理学院,江苏,南京,210009 |
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| 摘 要： | 目的利用反相高效液相色谱法同时测定莪术油注射液中莪术醇和牛儿酮含量。方法采用AgilentTC ODS色谱柱(4.6 mm×15 cm,5μm),流动相A:乙腈-甲醇-水-磷酸(550∶225∶225∶1);流动相B:甲醇,梯度洗脱,流速1.0 mL/min,柱温:35℃,检测波长:210 nm。结果在该条件下,莪术醇、牛儿酮浓度分别在0.940 6~94.06μg/mL和1.333~133.2μg/mL范围内与峰面积呈良好的线性关系;回收率分别为100.70%和100.37%,RSD分别为0.86%和0.92%。结论本法可用于同时测定莪术油注射液中莪术醇和牛儿酮的含量,方法简便、结果准确。
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| 关 键 词： | 莪术油注射液 莪术醇 牛儿酮 反相高效液相色谱法 |
| 文章编号： | 1005-1678(2007)06-0394-03 |
| 修稿时间： | 2007年4月25日 |
Determination of curcumol and germacrone in Zedoary Turmeric Oil Injections by RP-HPLC |
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| CAO Ling,WAN Rong,WANG Yu.Determination of curcumol and germacrone in Zedoary Turmeric Oil Injections by RP-HPLC[J].Chinese Journal of Biochemical Pharmaceutics,2007,28(6):394-396. |
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| Authors: | CAO Ling WAN Rong WANG Yu |
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| Abstract: | Purpose To develop a HPLC method for the determination of curcumol and gemacrone in Zedoary Turmeric Oil Injections.Methods The HPLC system consisted of a Agilent TC ODS column(4.6 mm×15 cm,5 μm)and DAD detecter at 210 nm,the flow rate was 1.0 mL/min,and the mobile phase was composed ofmobile phase A:acetonitrile-methanol-water-phosphoric acid(550∶225∶225∶1) and mobile phase B:methanol as gradient mode.Results It shows a good linear range of 0.940 6～94.06 μg/mL(curcumol)and 1.333～133.2 μg/mL(germacrone).The recoveries of curcumol and gemacrone were 100.70% and 100.37% repectively.Conclusion The method is appropriate for the determination of curcumol and gemacrone in Zedoary Turmeric Oil Injections. |
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| Keywords: | Zedoary Turmeric Oil Injections curcumol germacrone RP-HPLC |
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