膀胱癌可溶性抗原诱导细胞毒T细胞的实验研究

目的 观察膀胱癌可溶性抗原诱导细胞毒Ｔ细胞生长，为构建膀胱癌疫苗及过继性免疫治疗提供实验基础。方法 提膀胱癌可溶性抗原（ＴＳＡ），用ＴＳＡ、抗ＣＤ－３单抗、ＩＬ－２联合诱导外周血单个核细胞（ＰＢＭＣ），动态观察细胞增殖，进行细胞表型分析和细胞因子测定；并与ＩＬ－２诱导的ＬＡＫ细胞，抗ＣＤ－３单抗、ＩＬ－２诱导的ＣＤ３－ＡＫ细胞进行比较。结果 实验组细胞第８ｄ增殖７．５２倍，第１２ｄ增殖２８．９２倍

EXPERIMENTAL STUDY ON CYTOTOXIC T LYMPHOCYTES INDUCED BY SOLUBLE ANTIGEN OF BLADDER TUMOR

Objective To observe cytotoxic T lymphocytes induced by soluble antigen of bladder carcinoma for the development of tumor vaccine and the application of adoptive immunotherapy for bladder tumor. Methods Tumor soluble antigen (TSA) was extracted from bladder carcinoma. Peripheral blood mononuclear cells (PBMC) were co stimulated by TSA,IL 2 and anti CD3 monoclonal antibody to develop into cytotoxic T lymphocytes. The proliferation, phenotype and cytokine of the CTL were dynamically analysed and compared with LAK and CD3 AK. Results The proliferation folds of CTL were 7.52, 28.92, 32.8 and 36.96 on the days of 8th,12th,16th and 20th respectively; those of LAK were 5.72 and 0.82 on 12th and 20th, and those of CD3 AK were 24.46 and 27.72 on 12th and 20th respectively. The phenotype of CTL showed 16% CD4 , 89% CD8 and the cytotoxicity of the supernatant of CTL against L929 cell was 85.08% on the 8th day. Conclusion Soluble antigen of bladder tumor can induce PBMC into CTL which proliferate more rapidly and produce more TNF.