IL-6体外诱导成年大鼠骨髓破骨细胞形成的实验观察

目的 :探讨IL - 6对成年大鼠骨髓破骨细胞生成诱导的影响。方法 :采用 12周龄SD大鼠无菌条件下取出股骨 ,剪去两骺端 ,以α -MEM将骨髓细胞冲出 ,然后将细胞悬液接种于预置盖玻片或牙本质片的 2 4孔培养板内 ,次日实验组换含IL - 6 (10U/ml)的α -MEM培养液继续培养 2周 ,对照组培养液不含IL - 6。结果 :培养一周左右光镜下观察实验组可见明显单核细胞融合成多核细胞现象 ,多核细胞数量增多。经TRAP染色呈阳性反应 ,电镜下可观察到典型的破骨细胞骨吸收现象。结论 :IL - 6 (10U/ml)可明显诱导成年大鼠骨髓破骨细胞的形成

An experimental study of interleukin-6 (IL-6) induces osteoclast formation in adult rat bone marrow culture.

Objective:To investigate the effect of IL-6 on the osteoclastformation in adult rat bone marrow culture.Methods:The femur were removed aseptically from adult SD rat (aged 12 weeks).Both the epiphyseal ends of the femur were cut off with scissors,the marrow cavities were flushed with α-MEM.The suspended marrow cells were planted in 24-well tissue culture plates with cover-slips or dentin slices.Next day,the experimental groups media were replaced with α-MEM containing IL-6 (10U/ml) for 2 weeks.Results:For about 7days,Mononuclear cells fused to form multinuclear cell (MNC) in experimental groups based on the observation of viable cell morphology.MNC were postive in TRAP staining and could form typical resorption pits is SEM.Conclusions:It has been confirmed that IL-6(10U/ml) could induce osteoclast formation in adults rat bone marrow culture.