大鼠内淋巴囊的解剖学与组织学观察

目的 研究大鼠内淋巴囊的解剖学与组织学特征。方法 选用健康成年SD大鼠，处死后取双侧颞骨，先观察颞骨内侧面的解剖结构、内淋巴囊的位置及毗邻关系，然后将标本固定、脱钙、石蜡包埋切片，HE染色，观察内淋巴囊的形态和组织结构；再用抗大鼠IgG、CD3单克隆抗体，应用免疫组织化学技术，观察免疫活性细胞在内淋巴囊组织的定位分布。结果 大鼠内淋巴囊的上皮类型以单层立方上皮和扁平上皮为主；内淋巴囊上皮层、上皮下及囊周组织有淋巴细胞，CD3、IgG免疫反应阳性淋巴细胞主要存在于内淋巴囊的上皮层。结论 大鼠内淋巴囊具有与人和豚鼠内淋巴囊基本相似的形态结构；组织中存在CD3、IgG免疫活性细胞，提示其在内耳免疫应答过程中可能发挥重要作用。

THE STUDY ON ANATOMICAL AND HISTOLOGICAL FEATURES OF THE RAT ENDOLYMPHATIC SAC

Objective To investigate the anatomical and histological features of the rat endolymphatic sac. Methods The temporal bones of the healthy,adult SD rats were removed.The morphology of the endolymphatic sac were studied.Then the decalcified samples were embeded in paraffin.The sections of the endolymphatic sac were stained with HE method and observed histologically,and then examined immunohistochemically by monoclonal antibodies against IgG,CD 3 of the rat to investigate the immunocompetent cells in endolymphatic sac of the rats. Results Simple cuboidal and squamous epithelium were the main types of the epithelium in rat endolymphatic sac CD + 3,IgG + immunoreactive lymphocytes were found mainly in the epithelium of the endolymphatic sac.Conclusion The morphology of the rat endolymphatic sac is similar to those of human and guinea pig endolymphatic sacs.Some immunocompetent cells were found in the endolymphatic sac,suggesting that the endolymphatic sac plays an important role in immune of inner ear.;[