| 佛波醇酯对MES 23.5细胞铁调节蛋白1表达的影响 |
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| 引用本文： | 王文杰,宋宁,谢俊霞,王俊.佛波醇酯对MES 23.5细胞铁调节蛋白1表达的影响[J].青岛大学医学院学报,2011,47(5):379-381. |
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| 作者姓名： | 王文杰 宋宁 谢俊霞 王俊 |
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| 作者单位： | 青岛大学国家生理学重点,培育,学科,山东青岛266071 |
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| 基金项目： | 国家自然科学基金资助项目，山东省教育厅资助项目，青岛市科技局项目 |
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| 摘 要： | 目的探讨蛋白激酶C(PKC)激动剂佛波醇酯(PMA)对MES 23.5细胞中铁调节蛋白1(IRP1)的激活作用。方法采用MTT法观察细胞的存活率,Western Blot法检测IRP1和铁转出蛋白(Fpn1)的表达变化。结果 0.01~2.00μmol/L的PMA作用MES 23.5细胞24 h后,细胞存活率无明显变化(F=3.050,P>0.05)。0.20μmol/L的PMA作用MES 23.5细胞24 h后,IRP1表达明显上调(t=4.501,P<0.01),Fpn1表达明显下调(t=5.174,P<0.01)。结论 PMA在多巴胺能神经细胞系MES 23.5细胞中能明显激活IRP1。
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| 关 键 词： | 佛波醇酯类 神经元 铁调节蛋白质1 铁转出蛋白 |
THE EFFECT OF PMA ON THE ACTIVATION OF IRON REGULATORY PROTEIN-1 IN MES 23.5 CELLS |
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| WANG WEN-JIE,SONG NING,XIEJUN-XIA,WANG JUN.THE EFFECT OF PMA ON THE ACTIVATION OF IRON REGULATORY PROTEIN-1 IN MES 23.5 CELLS[J].Acta Academiae Medicinae Qingdao Universitatis,2011,47(5):379-381. |
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| Authors: | WANG WEN-JIE SONG NING XIEJUN-XIA WANG JUN |
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| Institution: | (State Key Disciplines: Physiology,Qingdao University Medical College,Qingdao 266071,China) |
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| Abstract: | Objective To study the activation of phorbol ester,agonist of protein kinase C(PKC) on iron regulatory protein-1(IRP1) in dopaminergic cell line MES 23.5 cells. Methods Cell viability was assessed by MTT.IRP1 and Fpn1 levels were investigated by Western Blot. Results After treatment with 0.01-2.00 μmol/L PMA for 24 hours,the survival of MES23.5 cells was not changed(F=3.050,P>0.05).After treatment with 0.20 μmol/L PMA for 24 hours,the expression of IRP1 upregulated(t=4.501,P<0.01),and Fpn1 expression down-regulated(t=5.174,P<0.01). Conclusion IRP1 can be activated by PMA in dopaminergic MES 23.5 cells. |
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| Keywords: | phorbol esters neurons iron regulatory protein 1 ferroprotein 1 |
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