荧光原位杂交技术研究慢性粒细胞白血病隐匿型变异易位

目的:探讨荧光原位杂交(FISH)技术对确定慢性粒细胞白血病(CML)隐匿型变异易位的重要作用。方法:骨髓细胞短期培养,常规显带用G显带技术,FISH检测以染色体全长涂抹探针进行。结果:485例CML患者中4例经常规G显带技术未见典型Ph染色体,FISH检出具有隐匿型Ph染色体(0．9％)。结论:FISH技术较传统显带技术准确、快速、特异、敏感,能准确辨认变异易位的类型,给予准确的分子细胞遗传学诊断,在CML的诊断和疗效监测中具有重要意义。

Application of fluorescence in situ hybridization in detecting Ph-masked variant translocation in chronic myeloid leukemia

Objective:To evaluate fluorescence in situ hybridization (FISH) in making judgement of Ph-masked variant translocation in chronic myeloid leukemia (CML). Method: Chromosome specimens were prepared by 24-hour culture of bone marrow cells, G-banding was used to do conventional cytogenetical analysis, and Whole Chromosome Painting probes (WCP) were used to do FISH. Result:Four in 485 CML cases (0. 9%) were not detected with typical Ph chromosome by G-banding, but with Ph-masked variant translocation by FISH. Conclusion:FISH is more rapid, specific and sensitive than conventional cytogenetic method and can be applied to identify type of variant translocation in CML. FISH is important in the diagnosis and treatment monitoring in CML.