卡托普利、地尔硫卓、多巴酚丁胺对主动脉血管内皮细胞单层通透性的影响

目的：探讨血管紧张素转换酶抑制剂卡托普利、钙离子拮抗剂地尔硫　和β－激动剂多巴酚丁胺对大鼠主动脉血管内皮细胞单层通透性的影响。方法：大鼠主动脉内皮细胞（ＡＥＣ）培养采用陈思锋的无创性分离法分离培养；将ＡＥＣ培养在混合纤维素酯微孔滤膜上，培养８ｄ后进行通透性测定。长有ＡＥＣ的滤膜分别用卡托普利、地尔硫　和多巴酚丁胺处理４ｈ，置于灌注装置上，用含异硫氰酸荧光素标记白蛋白的高脂血灌注，测定收集液的量和白蛋白浓度，同时采用酶联法测定滤出液的总胆固醇（Ｔｃｈ）、甘油三酯（ＴＧ）、载脂蛋白Ａ和Ｂ（ａＰＡ、ａＰＢ）浓度。结果：三种药物均可降低ＡＥＣ对水、Ｔｃｈ、ＴＧ、ａＰＡ和ａＰＢ的通透性，其中多巴酚丁胺作用最为显著。而对白蛋白清除率除地尔硫　作用较显著外，其他两药均无明显作用。结论：卡托普利、地尔硫　和多巴酚丁胺可减少脂类和载脂蛋白渗入血管内皮下，从而预防和改善动脉粥样硬化。

The effects of captopril,diltiazem and dobutamine on permeability of aortal endothelial cell monolayers

To investigate the effects of angiotensin converting enzyme inhibitor captopril, calcium channel blocher diltiazem and beta- adrenoceptor agonist dobutamine on the permeability of rat cultured aortic endothelial monolayers. Methods: The isolation and culture of rat aortic endothelial cells adopted the method of Chen SF. Rat aortic endothelial cells were seeded on the nitrocellulose microporous filters. 8 d after seeding, the monolayers were used for measuring the permeability by mounting monolayers. Before perfused, Monolayers were treated by captopril, diltiazem and dobutamine for 4 h successively. The prepared filters were mounted on the Boydon chambers and perfused with hyperlipemia containing FITC-labelled albumir. The fluid filtering through the monolayer and filter was collected and the albumin concentration were measured. At the same time Tch, TG, aPA and aPB concentrations of the collected fluid were also measured by ELISA. Results: The above three drugs decreased the permeability of confluent aortic endothelial cells monolayers to water, cholesterol, triglyceride lipoprotein A and lipoprotein B significantly. Dobutamine had more significant effects than two other drugs. Diltiazem, but not two other drugs decreased the clearance of albumin obviously. Conclusion: Captopril, diltiazem and dobutamine may decrease the infiltration of lipids and lipoproteins into the subendothelial space, thus they can be used to prevent and ameliorate atherosclerosis.