参七合剂对高脂血症大鼠的降脂效应及其机制

目的:使用自制高脂饲料,建立稳定的高脂血症大鼠模型.评价参七合剂的降脂效应,探讨其降脂机制.方法:将120只SD大鼠随机分成对照组(30只)和造模组(90只),使用自制高脂饲料喂养造模组SD大鼠,于第11天采血,检测血脂4项指标.将造模组大鼠先按血脂水平分层,再随机分为模型组、辛伐他汀组(0.001 g·kg-1)、参七合剂组(1 g·kg-1),每组30只,继续喂养20 d.第31天,采血,检测血脂4项,检测血液流变学指标,肝脏切片,HE染色,观察肝脏组织病理变化.结果:造模组SD大鼠第11天血脂4项指标结果显示造模成功.第31天血脂4项指标、血液流变学指标及肝细胞病理变化结果显示,参七合剂和辛伐他汀均能降低总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C) (P ＜0.01),升高高密度脂蛋白(HDL-C) (P ＜0.01),减轻大鼠肝细胞脂肪变性,降低血液黏度.与辛伐他汀比较,参七合剂降低TC,升高HDL-C,减轻肝细胞脂肪变性有显著差异.结论:采用高脂饲料喂养SD大鼠,建立了稳定的高血脂症动物模型.参七合剂能降低高血脂症模型大鼠的血脂水平及血液黏度,减轻肝细胞脂肪变性.

Experimental Study of Shenqi Mixture's Lipid-lowering Effect and Mechanism on Hyperlipidemia Rats

Objective: To establish stable hyperlipidemia animal model with self-designed high-fat diet. To evaluate lipid-lowering effect of Shenqi Mixture and investigate lipid-lowering mechanism of Shenqi Mixture. Method: One hundred and twenty SD rats were randomly divided into control group(30)and model group(90).The SD rats of model group were fed with self-designed high-fat diet. Hyperlipidemia models were evaluated successfully by testing four indexes of blood lipids after 10 days. Model rats were divided on lipid level and randomly divided into model group(1 g·kg^-1), Shenqi Mixture group and Simvastatin group(0.001 g·kg^-1)(30 each) orally for 20 days. Four indexes of blood lipids and hemorheology indexes were measured and pathological change of liver tissue was observed on 31^th day. Result: Four indexes of blood lipid of model SD rats' showed that the hyperlipidemia rats models were successful made on 11^th day. The four indexes of blood lipid,hemorheology indexes and pathological change of liver tissue showed that Shenqi Mixture and Simvastatin could decrease total cholesterol(TC), triglyceride(TG), low-density lipoproteins cholesterol(LDL-C)and blood viscosity,increase high-density lipoproteins cholesterol(HDL-C),and alleviate hepatic steatosis of liver tissue on 31^th day. The results of TC, HDL-C and hepatic steatosis of liver tissue in Shenqi Mixture group were significant different from Simvastatin group. Conclusion: Stable hyperlipidemia rat model can be established with self-designed high-fat diet. Shenqi Mixture can decrease lipid level and blood viscosity,alleviated hepatic steatosis of liver tissue.