运动对肥胖大鼠肌因子Irisin及骨骼肌AMPK的影响

目的 观察运动对肥胖大鼠血清Irisin及骨骼肌腺苷酸活化蛋白激酶(AMPK)水平的影响.方法 将50只大鼠随机分为正常组(n=10)、肥胖组(n=20)、肥胖运动组(n=20),通过高脂饲料喂养肥胖组及肥胖运动组大鼠以建立肥胖大鼠模型,模型建立成功后各筛选10只,对肥胖运动组大鼠进行6周的游泳运动,随后将动物处死取血样及骨骼肌组织,ELISA法检测各组大鼠血清Irisin的水平,Western blot法检测骨骼肌AMPK相关蛋白的表达.结果 肥胖组大鼠血清Irisin水平较正常组大鼠降低,骨骼肌AMPK磷酸化水平减弱(P＜0.01),而肥胖运动组大鼠血清Irisin水平较肥胖组大鼠增高,骨骼肌AMPK磷酸化水平增多(P＜0.01),各组大鼠血清Irisin水平与骨骼肌AMPK呈正相关性(P＜0.01).结论 运动能增强肥胖组大鼠血清Irisin水平及骨骼肌AMPK磷酸化水平,且两者呈正相关性,Irisin可能是运动效应分子机制的核心.

Effects of exercise on Irisin levels and skeletal muscle AMPK phosphorylation in obesity SD rats

Objective To investigate the effects of exercise on the serum Irisin and skeletal muscle AMPK of obese rats.Methods The rats were divided into normal group(n =10) and obesity group(n =20),exercise group(n =20).The obesity and exercise group were feeding with high fat feed to establish obesity model,and then the exercise group swimming for 6 weeks.Animals were then sacrificed to take blood samples and skeletal muscle tissue.ELISA assay was used to detect each group rats serum Irisin level,Western blot was used to detect the expression of skeletal muscle AMPK protein.Results The results showed that the serum Irisin level and skeletal muscle AMPK phosphorylation level of obese rats decreased compared with the normal group(P ＜ 0.01),while exercise group rats had higher serum Irisin levels and higher skeletal muscle AMPK phosphorylation level(P ＜ 0.01),there was a positive correlation between serum Irisin level and skeletal muscle AMPK,the differences were statistically significant (P ＜0.01).Conclusion Exercise could strengthen the serum Irisin level and skeletal muscle AMPK phosphorylation level on obese rats,and there is a positive correlation between them.Irisin may be the core of the molecular mechanism of exercise effects.