Electron microscopic radioautographic study on nucleic acid synthesis in perinatal mouse kidney tissue |
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Authors: | Toru Hanai and Tetsuji Nagata |
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Affiliation: | (1) Department of Anatomy and Cell Biology, Shinshu University School of Medicine, 3-1-1, Asahi, 390 Matsumoto, Japan |
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Abstract: | The kidney tissues of ddY mice from fetal to postnatal day 14 were labeledin vitro with either3H-thymidine or3H-uridine and analyzed by light and electron microscopic radioautography. The labeling index (L.I.) with3H-thymidine or grain-number-per-cell with3H-uridine was calculated by light microscopic radioautography. The L. I. of glomeruli and uriniferous tubules in the metanephric cortex were high throughout the embryonic stages, and decreased rapidly after birth. Many3H-thymidine labeled immature cells were observed in the embryonic stages by electron microscopic radioautography. The grain-number-per-cell of glomeruli and uriniferous tubules labeled with3H-uridine decreased gradually from fetal to postnatal stages. |
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Keywords: | Mouse Kidney Radioautography Nucleic acid synthesis Development |
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