抗菌钛合金Ti6Al4V-6Cu表面构建成骨细胞膜片的实验研究

目的:探讨大鼠颌骨成骨细胞在抗菌钛合金Ti6Al4V-6Cu表面构建细胞膜片的可行性。方法:体外培养大鼠颌骨成骨细胞，采用富含维生素C培养基在抗菌钛合金Ti6Al4V-6Cu表面构建细胞膜片（细胞膜片组），并以单纯培养基作对照（对照组），检测膜片形成过程中碱性磷酸酶（ALP）和成骨相关基因ALP、I型胶原（Col-1）、骨形成蛋白2（BMP-2）的表达情况。结果:采用富含维生素C的培养基连续培养可在抗菌钛合金Ti6Al4V-6Cu表面成功构建成骨细胞膜片，该细胞膜片由多层细胞构成，富含胞外基质。相对于对照组，细胞膜片组的膜片形成过程中成骨细胞ALP活性及成骨相关基因ALP、Col-1、BMP-2的表达均显著增高。结论:在抗菌钛合金Ti6Al4V-6Cu表面可成功构建成骨细胞膜片，有望与Ti6Al4V-6Cu联合应用于引导性骨再生术。

Construction of osteoblast membrane sheet on thesurface of antibacterial titanium alloy Ti6Al4V-6Cu

Objective To study the feasibility of constructing rat jaw osteoblast membrane sheet on the surface of antibacterial titanium alloy Ti6Al4V-6Cu. Methods Osteoblasts were isolated and cultured in vitro from jaw bone of rats firstly. Osteoblast membrane sheets were then constructed on the surface of the antibacterial titanium alloy Ti6Al4V-6Cu by using vitamin C-rich medium, and with those using pure medium as the control group, the expression of alkaline phosphatase (ALP) and osteoblast-related genes ALP, type I collagen (Col-1), and bone morphogenetic protein (BMP-2) was detected and analyzed. Results Osteoblast membrane sheet can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6Cu by continuous culture using vitamin C-rich medium. The osteoblast membrane sheet was composed of multiple layers of osteoblasts and rich in extracellular matrix. Compared with the control group, the ALP activity of osteoblasts and the expression of osteoblast-related genes ALP, Col-1, BMP-2 were significantly increased in the osteoblast membrane sheet group during formation. Conclusions Osteoblast membrane sheets can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6Cu, so it is hopeful to combinedly apply Ti6Al4V-6Cu to guided bone regeneration.