CFL1的表达对前列腺癌进展影响的研究

目的:探讨CFL1在前列腺癌中的表达情况，并明确其对前列腺癌进展的影响。方法:采用免疫组化、qPCR及Western blot检测CFL1的表达情况，采用Transwell实验检测细胞的迁移及侵袭能力，CCK-8实验检测细胞的增殖能力。结果:在恶性程度高的前列腺癌中，CFL1的蛋白及mRNA表达水平最高，恶性程度低的次之，良性前列腺增生最低（P＜0.05）；Transwell实验表明si-CFL1组较si-NC组，其迁移和侵袭能力均降低（均P＜0.05）；CCK-8实验显示敲低CFL1后，前列腺癌细胞的增殖能力降低（P＜0.05）。结论:CFL1在前列腺癌中高表达，而减低其表达水平可以抑制前列腺癌的进展。

Effect of CFL1 expression on prostate cancer progression

Objective: To investigate the expression of CFL1 in prostate cancer and to determine its effect on the progression of prostate cancer. Methods: The immunohistochemistry, qPCR and Western blot were used to detect the expression of CFL1. Transwell assay was used to detect the migration and invasion ability of cells. CCK-8 assay was used to detect the proliferation of cells. Results: In prostate cancer with high malignancy, CFL1 had the highest protein and mRNA expression levels, followed by low malignancy and benign prostatic hyperplasia（all P＜0.05）. Transwell experiments showed that the migration and invasion ability were decreased in si-CFL1 group compared with si-NC group(P＜0.05). The CCK-8 experiment showed that the proliferation of prostate cancer cells was decreased after CFL1 knockdown(P＜0.05). Conclusion: CFL1 is highly expressed in prostate cancer, and reducing its expression can inhibit the progression of prostate cancer.