基于槲皮素标记的金属有机框架放疗联合阿霉素诱导宫颈癌HeLa细胞凋亡的研究

目的探讨基于槲皮素(Quercetin,QU;3,3′,4′,5,7-五羟基黄酮)标记的金属有机框架(Zr metal organic frameworks,Zr-MOF)放疗(Radiation therapy,RT)联合阿霉素(Doxorubicin,DOX)对宫颈癌HeLa细胞增殖和凋亡的影响。方法将宫颈癌HeLa细胞分为对照组、RT组、阿霉素处理组(Zr-MOF-DOX+RT)、QU处理组(Zr-MOF-QU+RT)及联合处理组(Zr-MOF-QU-DOX+RT);采用细胞克隆形成法检测各处理组HeLa细胞的增殖活性;蛋白印迹法及免疫荧光法检测各组细胞凋亡蛋白Caspase-3及缺氧诱导因子1α(Hypoxia inducible factor-1α,HIF-1α)的表达水平。结果联合处理组细胞的增殖能力明显低于其他各组,差异具有统计学意义(P<0.05);联合处理组HIF-1α蛋白表达量明显低于其他各组(P<0.05),联合处理组Caspase-3蛋白的表达含量明显高于其他各组(P<0.05)。结论基于槲皮素标记的金属有机框架放疗联合阿霉素对HeLa细胞的抑...

Quercetin-labeled metal organic framework radiotherapy combined with adriamycin induces apoptosis of cervical cancer HeLa cells

Objective The aim of this study was to explore the radiation therapy(RT)of modified metal organic frameworks(Zr metal organic frameworks,Zr-MOF)based on quercetin(QU;3,3 ′,4 ′,5,7-5 hydroxyflavone)combined with doxorubicin(DOX)on the proliferation and apoptosis of cervical cancer HeLa cells.Methods HeLa cells were divided into the control group,RT group,doxorubicin treatment group(Zr-MOF-DOX+RT),QU treatment group(Zr-MOF-QU+RT),and combined treatment group(Zr-MOF-QU-DOX+RT).The cell clone formation assay was used to detect proliferation of HeLa cells in each treatment group.Western blot and immunofluorescence assays were used to detect the expression of apoptotic protein Caspase-3 and hypoxia inducible factor-1α(HIF-1α)in HeLa cells.Results The cell proliferation in the combined group was significantly lower than that of the other groups,and the difference was statistically significant(P＜0.05).The expression of HIF-1α protein in the combination group was significantly lower than that in the other groups(P＜0.05),and the expression of Caspase-3 protein in the combination group was significantly higher than that in the other treatment groups(P＜0.05).Conclusion Quercetin-labeled metal organic framework radiotherapy combined with doxorubicin has a more significant inhibitory effect on HeLa cells.The combined treatment group has a strong effect on antitumor by up-regulating the expression of apoptotic protein Caspase-3 and down-regulating HIF-1α.