紫檀芪通过PI3K/Akt通路对脓毒症大鼠膈肌的保护作用

目的：研究紫檀芪（PTS）对脓毒症致大鼠膈肌损伤的作用及其可能机制。方法：通过盲肠结扎穿刺术建立大鼠脓毒症模型。将大鼠随机分为3组：假手术组、脓毒症组和PTS组（25 mg/kg），24 h后处死大鼠，采集膈肌进行组织病理学检查及膈肌损伤评分（DDS）；TUNEL染色法检测膈肌组织细胞凋亡；ELISA法测定膈肌组织中IL-1β、TNF-α、IL-6、AST、LDH、CK及MPO含量；Western blot检测膈肌组织中p-PI3K和p-Akt的表达。结果：与假手术组相比较，脓毒症组中DDS评分、细胞凋亡率、IL-1β、TNF-α、IL-6、AST、LDH、CK及MPO含量均明显升高，而p-PI3K和p-Akt蛋白含量下调，差异有统计学意义（P ＜0.05）。相比脓毒症组，PTS组中DDS评分、细胞凋亡率、IL-1β、TNF-α、IL-6、AST、LDH、CK及MPO含量均明显下调，而p-PI3K和p-Akt蛋白含量上调，差异有统计学意义（P ＜0.05）。结论：PTS通过PI3K/Akt途径抑制脓毒症致大鼠膈肌损伤。

The protective effect of pterostilbene on rat diaphragm during sepsis via PI3K-Akt pathway

Objective: To explore the protective effect of pterostilbene (PTS) on sepsis-induced diaphragm injury and its possible mechanism in a rat model. Methods: Sepsis model was induced by cecal ligation and puncture (CLP) in rats. The rats, randomly divided into three groups: sham group, CLP group and PTS groups(25 mg/kg), were killed after CLP for 24 h. Diaphragm tissues were collected for pathological analysis and diaphragm Damage Score (DDS) detection. The apoptosis of diaphragm tissues was evaluated using TUNEL assay. The content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in diaphragm specimen was measured by ELISA assay. Western blot was used to explore the level of p-Akt and p-PI3K in diaphragm tissues. Results:Compared with sham group, the DDS score, apoptotic rate and the content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in CLP group were all promoted markedly. The level of p-Akt and p-PI3K was reduced during sepsis.However, the DDS score, apoptotic rate and the content of IL-1β, TNF-α, IL-6, AST, LDH, CK and MPO in CLP group were all markedly decreased by the administration of PTS, while the expression of p-Akt and p-PI3K was both increased after PTS treatment. Conclusion: PTS plays a protective role in diaphragm via PI3K/Akt pathway in a CLP-induced sepsis rat model, which provides a candidate for drug therapy of sepsis-induced diaphragm injury.