牙周炎孕妇龈沟液及血清中MMP-9和MMP-25检测对早产的预测价值

目的 初步评估牙周炎孕妇龈沟液及血清中基质金属蛋白酶-9,25(MMP-9,MMP-25)的表达水平对早产的预测价值,探讨孕妇牙周炎对妊娠结局的影响。方法 以62例牙周炎孕妇及60例牙周组织健康的孕妇为研究对象,留取受试者的龈沟液和血清,采用酶联免疫吸附法检测MMP-9、MMP-25的表达水平,比较两组间MMP-9和MMP-25表达差异,并比较孕妇牙周状况和分娩孕周数之间的关系。结果 牙周炎组孕妇分娩孕周显著小于对照组(P<0.05);与对照组相比,牙周炎组孕妇龈沟液及血清中MMP-9和MMP-25表达水平显著升高 (P<0.05)。结论 牙周炎孕妇龈沟液和血清中MMP-9和MMP-25的表达水平显著升高,分娩孕周缩短。在妊娠中晚期检测孕妇体液中MMP-9和MMP-25表达水平可作为降低早产发生率的干预靶点。

Detection effect of gingival crevicular fluid and serum matrix metalloproteinases in pregnant women with periodontitis on preterm birth prediction

Objective To evaluate the predictive value of matrix metalloproteinases in preterm labor in pregnant women with periodontitis and examine the hypothesis that periodontitis was one of the sources of maternal inflammation in premature infants. Methods The samples of gingival crevicular fluid (GCF) and serum were collected from groups of 62 periodontitis mothers (PG) and 60 oral healthy mothers (HG). Levels of MMP-9 and MMP-25 were analyzed by enzyme-linked immunosorbent assay (ELISA). Results The gestational weeks of pregnant women in the PG were significantly fewer than those in the HG (P<0.05). MMP-9 and MMP-25 expression levels in GCF and serum were significantly higher in the PG than in the HG (P<0.05). Conclusion There was a tendency presenting the higher expression of MMP-9 and MMP-25 in CGF and serum, the shorter gestational period. Detection of the expression level of MMP-9 and MMP-25 in the body fluids of pregnant women with periodontitis in middle and late pregnancy can be used as a target to reduce the preterm rate.