| IL-17A可以促进卵巢癌的腹腔转移 |
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| 引用本文： | 李岩1,牛秀珑2,郁春艳1,陈燕1,沈洋洋1,邓为民1. IL-17A可以促进卵巢癌的腹腔转移[J]. 天津医科大学学报, 2020, 0(6): 518-520,525 |
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| 作者姓名： | 李岩1 牛秀珑2 郁春艳1 陈燕1 沈洋洋1 邓为民1 |
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| 作者单位： | 1.天津医科大学基础医学院免疫学系,国家教育部免疫微环境与疾病重点实验室,天津 300070;2.中国人民武装警察部队特色医学中心勤务环境皮肤疾病防治研究所,天津 300162 |
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| 摘 要： | 目的:探讨白细胞介素（IL）-17A对卵巢癌（OvCa）腹腔转移的影响。方法:以C57BL/6为遗传背景的野生型（WT）小鼠和IL-17A 缺陷（IL-17A-/-）小鼠为研究对象,腹腔注射同基因小鼠OvCa细胞系ID8,检测内源性IL-17A对OvCa腹腔种植瘤生长的影响。应用MTT法检测不同浓度的重组小鼠IL-17A（rmIL-17A）对ID8细胞增殖的影响;应用划痕实验检测不同浓度的rmIL-17A对ID8细胞迁移能力的影响;应用Transwell侵袭实验检测不同浓度的rmIL-17A对ID8细胞侵袭能力的影响。结果:与IL-17A-/-小鼠相比,WT小鼠腹腔内肠系膜、腹膜后淋巴结、脾脏和肾脏表面等处均见密集瘤结节转移灶,且腹腔内瘤结节数量显著增多（均P＜0.01）。体外实验结果显示,与对照组相比,不同浓度的rmIL-17A对ID8细胞增殖无影响（均P>0.05）;与对照组相比,10 ng/mL的rmIL-17A作用6 h后可促进ID8细胞迁移,1 ng/mL和10 ng/mL的rmIL-17A作用12 h后可显著促进ID8细胞迁移（均P＜0.05）;与对照组相比,干预24 h后,1 ng/mL和10 ng/mL的rmIL-17A对ID8细胞的侵袭能力均有促进作用（均P＜0.05）。结论:IL-17A可以促进卵巢癌的腹腔转移。
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| 关 键 词： | IL-17A 卵巢癌 转移 侵袭 |
IL-17A promotes intraperitoneal metastasis of ovarian cancer |
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| LI Yan,NIU Xiu-long,YU Chun-yan,CHEN Yan,SHEN Yang-yang,DENG Wei-min . IL-17A promotes intraperitoneal metastasis of ovarian cancer[J]. Journal of Tianjin Medical University, 2020, 0(6): 518-520,525 |
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| Authors: | LI Yan NIU Xiu-long YU Chun-yan CHEN Yan SHEN Yang-yang DENG Wei-min |
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| Affiliation: | 1.Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Key Laboratory of Diseases and Microenvironment of Ministry of Education of China, Tianjin 300070, China; 2.Department of Prevention, Therapy of Skin Disease in the Security Environment, Characteristic Medical Center of Chinese People′s Armed Police Force, Tianjin 300162, China |
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| Abstract: | Objective: To investigate the effect of interleukin-17A（IL-17A） on intraperitoneal metastasis of ovarian cancer（OvCa）. Methods: Wild-type (WT) mice and IL-17A-deficient (IL-17A-/-) mice with C57BL/6 genetic background were used as research subjects. The effect of endogenous IL-17A on OvCa intraperitoneal metastasis was evaluated by intraperitoneal injection of syngeneic mouse ovarian cancer cell line ID8. MTT assay was used to detect the effect of recombinant mouse IL-17A(rmIL-17A) on ID8 cells proliferation. Wound healing assay was used to detect the effect of rmIL-17A on ID8 cells migration. Transwell invasion assay was used to detect the effect of rmIL-17A on the invasion ability of ID8 cells. Results: Compared with IL-17A-/- mice, much denser tumor nodules were observed on the surface of mesentery, retroperitoneal lymph nodes, spleen and kidney in WT mice, and the number of nodules in the abdominal cavity was significantly increased (all P<0.01). In vitro results showed that rmIL-17A had no effect on ID8 cells proliferation. Compared with control group, rmIL-17A at 10 ng/mL could promote the migration of ID8 cells after 6 h, and rmIL-17A at 1 ng/mL and 10 ng/mL could significantly promote the migration of ID8 cells after 12 h (all P＜0.05). Compared with control group, after 24 hours of intervention, 1 ng/mL and 10 ng/mL rmIL-17A both promoted the invasion of ID8 cells(all P＜0.05). Conclusion: IL-17A can enhance the intraperitoneal metastasis of OvCa. |
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| Keywords: | IL-17A ovarian cancer metastasis invasion |
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