人类白细胞抗原DRA基因多态性与肝细胞癌遗传易感性的关联研究

目的 探讨人类白细胞抗原DRA基因（human leukocyte antigen DRA，HLA-DRA）单核苷酸多态性（single nucleotide polymorphism，SNP）与肝细胞癌（Hepatocellular carcinoma，HCC）易感性的关联。方法 采用以医院为来源的非匹配病例对照研究方法，纳入365例HCC患者作为病例组以及365例非HCC患者作为对照组。利用问卷收集研究对象的一般人口学特征和相关暴露因素资料，并采用TaqMan-MGB探针定量PCR法对rs3135395 G>T、rs3135338 T>C和rs2395178 G>C进行基因型检测。应用非条件logistic回归模型计算SNP位点各基因型与HCC易感性的比值比（odds ratio，OR）及95%可信区间（confidence interval，CI）。结果 携带rs3135338 TC基因型和显性模型（TC+CC）的个体HCC易感性较TT基因型携带者显著降低（OR=0.47，95% CI=0.25~0.91，P=0.024；OR=0.53，95% CI=0.28~0.95，P=0.045）。与rs2395178 GG基因型携带者相比，GC基因型和显性模型（GC+CC）携带者HCC易感性显著降低（OR=0.41，95% CI=0.29~0.57，P<0.001；OR=0.58，95% CI=0.41~0.81，P=0.002）。结论 HLA-DRA基因rs3135338 T>C和rs2395178 G>C与HCC易感性相关，可为揭示免疫相关基因遗传多态性对HCC发生的作用提供一定参考。

Association between Human leukocyte antigen DRA gene polymorphisms and susceptibility to hepatocellular carcinoma

Objective To investigatethe association between single nucleotide polymorphism (SNP) of human leukocyte antigen DRA (HLA-DRA) gene and susceptibility to hepatocellular carcinoma (HCC). WTHZ]Methods A hospital-based non-matched case-control study was conducted among 365 patients with HCC as a case group and 365 patients without HCC as a control group. The questionnaire was used to collect the general demographic characteristics and relevant exposure factors, and the TaqMan-MGB probe quantitative PCR method was performed to detect the genotypes of rs3135395 G>T, rs3135338 T>C, and rs2395178 G>C. The unconditional logistic regression model was used to calculate the odds ratio (OR) and 95% confidence interval (CI) of each genotype of SNP to HCC susceptibility. WTHZ]Results Subjects with rs3135338 TC genotype and dominant model (TC+CC) were significantly less susceptible to HCC than TT genotype carriers (OR=0.47, 95% CI=0.25-0.91, P=0.024; OR=0.53, 95% CI=0.28-0.95, P=0.045). Compared with rs2395178 GG genotype carriers, subjects with GC genotype and dominant model (GC+CC) had significantly lower HCC susceptibility (OR=0.41, 95% CI=0.29-0.57, P<0.001; OR=0.58, 95% CI=0.41-0.81, P=0.002). WTHZ]Conclusion HLA-DRA rs3135338 T>C and rs2395178 G>C are associated with HCC susceptibility, which can provide a reference for revealing the role of genetic polymorphisms of immune-related genes on the occurrence of HCC.